trFluor Tb羊抗鼠免疫球蛋白(H+L)*交叉亲和 降低干扰*

产品基本信息

产品名称：trFluor Tb羊抗鼠免疫球蛋白(H+L)*交叉亲和 降低干扰*

储存条件：-15℃避光防潮

保质期：24个月

 

产品物理化学光谱特性

分子量：N/A

溶剂：水

激发波长(nm)：330

发射波长(nm)：544

 

产品介绍

trFluor Tb羊抗鼠免疫球蛋白(H+L)是美国AAT Bioquest生产的荧光标记二抗，存在于细胞，血清或其他生物流体中的许多生物化合物都是天然荧光的，因此，由于要测定的生物分子的自发荧光引起的高背景，使用常规的快速荧光团会严重限制测定灵敏度。长寿命荧光团与时间分辨检测（激发和发射检测之间的延迟）结合使用，可大程度地减少即时荧光干扰。我们的trFluor Tb探针可用于需要高灵敏度的测定的时间分辨荧光（TRF）。与更传统的荧光团（例如Alexa Fluor或花青染料）相比，trFluor Tb探针具有较大的斯托克斯位移和极长的发射半衰期。与其他TRF化合物相比，我们的trFluor Tb探针具有相对较高的稳定性，高发射率和与生物分子连接的能力。当与一抗结合使用时，这种trFluor Tb山羊抗小鼠IgG（H + L）缀合物通常用作间接免疫荧光染色的第二步试剂。百萤生物是AAT Bioquest的中国代理商，为您提供优质的trFluor Tb羊抗鼠免疫球蛋白(H+L)。 

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参考文献

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Time-resolved fluorescence resonance energy transfer (TR-FRET) to analyze the disruption of EGFR/HER2 dimers: a new method to evaluate the efficiency of targeted therapy using monoclonal antibodies
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A time-resolved fluorescence-resonance energy transfer assay for identifying inhibitors of hepatitis C virus core dimerization
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Ligand regulation of the quaternary organization of cell surface M3 muscarinic acetylcholine receptors analyzed by fluorescence resonance energy transfer (FRET) imaging and homogeneous time-resolved FRET
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Steady-state and time-resolved fluorescence quenching with transition metal ions as short-distance probes for protein conformation
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