trFluor Eu羊抗兔免疫球蛋白(H+L)*交叉亲和 降低干扰*

产品基本信息

产品名称：trFluor Eu羊抗兔免疫球蛋白(H+L)*交叉亲和 降低干扰*

储存条件：-15℃避光防潮

保质期：24个月

 

产品物理化学光谱特性

分子量：N/A

溶剂：水

激发波长(nm)：346

发射波长(nm)：617

 

产品介绍

trFluor Eu羊抗兔免疫球蛋白(H+L)是美国AAT Bioquest生产的荧光标记二抗，存在于细胞，血清或其他生物流体中的许多生物化合物都是天然荧光的，因此，由于要测定的生物分子的自发荧光引起的高背景，使用常规的快速荧光团会严重限制测定灵敏度。长寿命荧光团与时间分辨检测（激发和发射检测之间的延迟）结合使用，可大程度地减少即时荧光干扰。我们的trFluor Eu探针可用于需要高灵敏度的测定的时间分辨荧光（TRF）。与更传统的荧光团（例如Alexa Fluor或花青染料）相比，trFluor Eu探针具有较大的斯托克斯位移和极长的发射半衰期。与其他TRF化合物相比，我们的trFluor Eu探针具有相对较高的稳定性，高发射率和与生物分子连接的能力。当与一抗结合使用时，这种trFluor Eu山羊抗兔IgG（H + L）缀合物通常用作间接免疫荧光染色的第二步试剂。百萤生物是AAT Bioquest的中国代理商，为您提供优质的trFluor Eu羊抗兔免疫球蛋白(H+L)。 

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参考文献

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Time-Resolved Fluorescence Resonance Energy Transfer as a Versatile Tool in the Development of Homogeneous Cellular Kinase Assays
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Journal: Assay Drug Dev Technol. (2012)

A homogeneous single-label time-resolved fluorescence cAMP assay
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Journal: J Biomol Screen (2011): 356

Homogeneous time-resolved fluorescence-based assay to screen for ligands targeting the growth hormone secretagogue receptor type 1a
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Journal: Anal Biochem (2011): 253

Oligomerization of the serotonin(1A) receptor in live cells: a time-resolved fluorescence anisotropy approach
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Journal: J Phys Chem B (2011): 11439

Time-resolved fluorescence resonance energy transfer (TR-FRET) to analyze the disruption of EGFR/HER2 dimers: a new method to evaluate the efficiency of targeted therapy using monoclonal antibodies
Authors: Gaborit N, Larbouret C, Vallaghe J, Peyrusson F, Bascoul-Mollevi C, Crapez E, Azria D, Chardes T, Poul MA, Mathis G, Bazin H, Pelegrin A.
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A time-resolved fluorescence-resonance energy transfer assay for identifying inhibitors of hepatitis C virus core dimerization
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Journal: Assay Drug Dev Technol (2010): 96

Ligand regulation of the quaternary organization of cell surface M3 muscarinic acetylcholine receptors analyzed by fluorescence resonance energy transfer (FRET) imaging and homogeneous time-resolved FRET
Authors: Alvarez-Curto E, Ward RJ, Pediani JD, Milligan G.
Journal: J Biol Chem (2010): 23318

Steady-state and time-resolved fluorescence quenching with transition metal ions as short-distance probes for protein conformation
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Journal: Anal Biochem (2010): 284

Time-resolved FRET fluorescence spectroscopy of visible fluorescent protein pairs
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Journal: Eur Biophys J (2010): 241

 

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