trFluor Eu羊抗鼠免疫球蛋白(H+L)

产品基本信息

产品名称：trFluor Eu羊抗鼠免疫球蛋白(H+L)

储存条件：-15℃避光防潮

保质期：24个月

 

产品物理化学光谱特性

分子量：N/A

溶剂：水

激发波长(nm)：346

发射波长(nm)：617

 

产品介绍

trFluor Eu羊抗鼠免疫球蛋白(H+L)是美国AAT Bioquest生产的荧光标记二抗，许多生物分子自身就具有荧光，它们广泛的存在于生物器官里，如细胞、浆液和其他生物制品中。因此传统的敏感荧光素存在严重的局限性和不足，在进行生物监测分析时，生物分子的自发荧光形成很高的背景干扰。解决的方法是用长寿命的荧光素轭合物它具有高的时间分辨率（延长激发波谱和发射波普的检测）大程度的减少背景荧光干扰。我们的 trFluor Eu荧光探针具有高灵敏度的能够做到时间分辨检测。在和传统的荧光探针相比（例如Alexa的Fluro和花箐染料）， trFluor Eu 荧光探针具有较大的斯托克斯位移和极其长的发射光谱半衰期。同另外的TRF化合物相比我们的 trFluor Eu荧光探针具有较高的稳定性和很高的量子产率，而且可以和生物分子相连接。这一 trFluor Eu  山羊-老鼠 IgG (H+L) 轭合物在连接生物素酰化的抗体时，通常用作第二步简接免疫荧光试剂。在以生物素莲亲和素为基础的生物学流式测试或者分析方面他是一个非常有用的工具。百萤生物是AAT Bioquest的中国代理商，为您提供优质的trFluor Eu羊抗鼠免疫球蛋白(H+L)。 

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参考文献

Development of a time-resolved fluorescence resonance energy transfer assay for cyclin-dependent kinase 4 and identification of its ATP-noncompetitive inhibitors
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Journal: Anal Biochem (2012): 368

Time-Resolved Fluorescence Resonance Energy Transfer as a Versatile Tool in the Development of Homogeneous Cellular Kinase Assays
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Journal: Assay Drug Dev Technol. (2012)

A homogeneous single-label time-resolved fluorescence cAMP assay
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Journal: J Biomol Screen (2011): 356

Homogeneous time-resolved fluorescence-based assay to screen for ligands targeting the growth hormone secretagogue receptor type 1a
Authors: Leyris JP, Roux T, Trinquet E, Verdie P, Fehrentz JA, Oueslati N, Douzon S, Bourrier E, Lamarque L, Gagne D, Galleyrand JC, M'Kadmi C, Martinez J, Mary S, Baneres JL, Marie J.
Journal: Anal Biochem (2011): 253

Oligomerization of the serotonin(1A) receptor in live cells: a time-resolved fluorescence anisotropy approach
Authors: Paila YD, Kombrabail M, Krishnamoorthy G, Chattopadhyay A.
Journal: J Phys Chem B (2011): 11439

Time-resolved fluorescence resonance energy transfer (TR-FRET) to analyze the disruption of EGFR/HER2 dimers: a new method to evaluate the efficiency of targeted therapy using monoclonal antibodies
Authors: Gaborit N, Larbouret C, Vallaghe J, Peyrusson F, Bascoul-Mollevi C, Crapez E, Azria D, Chardes T, Poul MA, Mathis G, Bazin H, Pelegrin A.
Journal: J Biol Chem (2011): 11337

A time-resolved fluorescence-resonance energy transfer assay for identifying inhibitors of hepatitis C virus core dimerization
Authors: Kota S, Scampavia L, Spicer T, Beeler AB, Takahashi V, Snyder JK, Porco JA, Hodder P, Strosberg AD.
Journal: Assay Drug Dev Technol (2010): 96

Ligand regulation of the quaternary organization of cell surface M3 muscarinic acetylcholine receptors analyzed by fluorescence resonance energy transfer (FRET) imaging and homogeneous time-resolved FRET
Authors: Alvarez-Curto E, Ward RJ, Pediani JD, Milligan G.
Journal: J Biol Chem (2010): 23318

Steady-state and time-resolved fluorescence quenching with transition metal ions as short-distance probes for protein conformation
Authors: Posokhov YO, Kyrychenko A, Ladokhin AS.
Journal: Anal Biochem (2010): 284

Time-resolved FRET fluorescence spectroscopy of visible fluorescent protein pairs
Authors: Visser AJ, Laptenok SP, Visser NV, van Hoek A, Birch DJ, Brochon JC, Borst JW.
Journal: Eur Biophys J (2010): 241

 

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