膜电位荧光探针TMA-DPH CAS 115534-33-3

膜电位荧光探针TMA-DPH是美国AAT Bioquest生产的用于检测膜电位的荧光探针，TMA-DPH是用于膜流动性测量的荧光探针。它在脂蛋白和类似类似系统的单层动力学研究中有用。DPH及其衍生物（例如TMA-DPH）是圆柱形的并且具有荧光发射转变偶极子，其基本上与它们的长分子轴平行排列。因此，它们对由与周围脂质的相互作用引起的重新定向非常敏感。它们广泛用于旋转运动的荧光偏振研究。百萤生物是AAT Bioquest的中国代理商，为您提供优质的膜电位荧光探针TMA-DPH。 

操作步骤

        以下程序适用于大多数细胞类型。生长培养基，细胞密度，其他细胞类型的存在和其他因素可能影响染色。玻璃器皿上的残留洗涤剂也可能影响许多生物的染色，导致在有或没有细胞存在的溶液中出现明亮染色的物质。

1）通过离心沉淀细胞，并将细胞重悬于缓冲盐溶液或培养基中，在pH7.4下具有佳染料结合。培养物中的贴壁细胞可以在盖玻片上或细胞培养孔中原位染色。
2）制备10-50mM的TMA-DPH DMSO储备溶液。
3）在Hanks和20mM Hepes缓冲液pH 7.4中使用0.5和5μM之间的浓度添加TMA-DPH染色，并在37℃，5％CO₂培养箱中孵育5分钟。在初的实验中，好在整个建议范围内尝试几种染料浓度，以确定产生佳染色的浓度。
4）洗涤细胞并重悬于适当的pH 7.4的Hepes缓冲液中。

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