Sulfide Green AM

产品基本信息

产品名称：Sulfide Green AM

储存条件：-15℃避光防潮

保质期：12个月

 

产品物理化学光谱特性

分子量：~700

溶剂：DMSO

激发波长(nm)：500

发射波长(nm)：522

 

产品介绍

Sulfide Green AM是用于检测活细胞中硫化氢的荧光探针。 对于活细胞中的硫化物阴离子或硫化氢成像，它具有细胞渗透性。Sulfide Green AM包含疏水性的乙酰氧基甲基酯基，使其具有细胞渗透性。 当Sulfide Green AM进入活细胞时，其AM部分被酯酶切割。 酯酶诱导的Sulfide Green AM水解产生带负电荷的荧光产物，该产物很好地保留在细胞中。 与硫化物或硫化氢反应时，硫化物Green产生与硫化物浓度成比例的强绿色荧光。 Sulfide Green AM激活后可检测细胞中低至nM的硫化物阴离子。百萤生物是AAT Bioquest的中国代理商，为您提供优质的Sulfide Green AM。 

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图示

图1.用SA7测量Na₂S剂量反应。 将Na₂S标准品在20mM Hepes缓冲液中连续稀释，将20mM Hepes缓冲液中的SF7加入每个孔中，以达到最终浓度10uM。 该测定在室温下孵育60分钟，然后测量Ex / Em ＝ 490 / 530nm，截止值＝ 515nm。

 

参考文献

Angiotensin II downregulates vascular endothelial cell hydrogen sulfide production by enhancing cystathionine gamma-lyase degradation through ROS-activated ubiquitination pathway
Authors: Bai, L., Qi, Y., Chen, S., Wang, J., Tang, C., Du, J., Jin, H., Huang, Y.
Journal: Biochem Biophys Res Commun (2019): 907-912

Construction of a novel cell-trappable fluorescent probe for hydrogen sulfide (H2S) and its bio-imaging application
Authors: Lin, X., Chen, Y., Wang, S., Liu, K., Kong, F.
Journal: Anal Bioanal Chem (2019): se name="21301.enl" path="C:\Users\aatbi\Dropbox (AAT Bioquest)\Website Working Files\Product References\21301.enl">21301.enlEndNote3317Lin, X.Chen, Y.Wang, S.Liu, K.Kong, F.State Key Laboratory of Biobased Material and Green Papermaking, Key Laboratory o

Corrigendum to "Synergistic growth inhibition of mouse skin tumors by pomegranate fruit extract and diallyl sulfide: Evidence for inhibition of activated MAPKs/NF-kappaB and reduced cell proliferation" [Food Chem. Toxicol. 49 (2011) 1511-20]
Authors: George, J., Singh, M., Srivastava, A. K., Bhui, K., Shukla, Y.
Journal: Food Chem Toxicol (2019): 110784

Deficiency of the mitochondrial sulfide regulator ETHE1 disturbs cell growth, glutathione level and causes proteome alterations outside mitochondria
Authors: Sahebekhtiari, N., Fern and ez-Guerra, P., Nochi, Z., Carlsen, J., Bross, P., Palmfeldt, J.
Journal: Biochim Biophys Acta Mol Basis Dis (2019): 126-135

Facile, rapid one-pot synthesis of multifunctional gold nanoclusters for cell imaging, hydrogen sulfide detection and pH sensing
Authors: Gao, P., Li, M., Zhang, Y., Dong, C., Zhang, G., Shi, L., Li, G., Yuan, M., Shuang, S.
Journal: Talanta (2019): 1-11

Haloalkaliphilic microorganisms assist sulfide removal in a microbial electrolysis cell
Authors: Ni, G., Harnawan, P., Seidel, L., Ter Heijne, A., Sleutels, T., Buisman, C. J. N., Dopson, M.
Journal: J Hazard Mater (2019): 197-204

Hydrogen Sulfide Attenuates High Glucose-Induced Human Retinal Pigment Epithelial Cell Inflammation by Inhibiting ROS Formation and NLRP3 Inflammasome Activation
Authors: Wang, P., Chen, F., Wang, W., Zhang, X. D.
Journal: Mediators Inflamm (2019): 8908960

Hydrogen sulfide donor GYY4137 suppresses proliferation of human colorectal cancer Caco-2 cells by inducing both cell cycle arrest and cell death
Authors: Sakuma, S., Minamino, S., Takase, M., Ishiyama, Y., Hosokura, H., Kohda, T., Ikeda, Y., Fujimoto, Y.
Journal: Heliyon (2019): e02244

Hydrogen sulfide enhances the effectiveness of mesenchymal stem cell therapy in rats with heart failure: In vitro preconditioning versus in vivo co-delivery
Authors: Abdelmonem, M., Shahin, N. N., Rashed, L. A., Amin, H. A. A., Shamaa, A. A., Shaheen, A. A.
Journal: Biomed Pharmacother (2019): 108584

Hydrogen sulfide-mediated regulation of cell death signaling ameliorates adverse cardiac remodeling and diabetic cardiomyopathy
Authors: Kar, S., Kambis, T. N., Mishra, P. K.
Journal: Am J Physiol Heart Circ Physiol (2019): H1237-H1252