ReadiUse 细菌细胞裂解液*5X*
| Ex (nm) | - | Em (nm) | - |
| 分子量 | N/A | 溶剂 | - |
| 存储条件 | 在2-8度冷藏保存 |
细胞裂解是指细胞的分解，通常用于分析特定的细胞组成，例如蛋白质、脂质、核酸、报告分子、细胞信号分子和其他小生物分子。根据所用的裂解液，全部或部分膜被溶解。ReadiUse 试剂需要少的动手时间，这种细菌细胞裂解缓冲液只需简单的5倍稀释即可。它广泛用于裂解细胞以定量细菌中的小生物分子，例如NAD（P）/ NAD（P）H。百萤生物是AAT Bioquest的中国代理商，为您提供优质的ReadiUse 细菌细胞裂解液。
样品实验方案
溶液配制
ReadiUse 细菌细胞裂解缓冲液工作液（1X）： 将1 mL ReadiUse 细菌细胞裂解缓冲液（5X）加入4 mL Mili-Q水中，并充分混合。
样品示例及操作
- 通过离心收集细菌细胞（10,000 g，0°C，15分钟）来制备细菌细胞样品。使用大约100至1000万个细胞/ mL的ReadiUse 裂解缓冲液工作溶液（1X），将处理过的溶液在室温下放置15分钟。
- 以2500 rpm离心5分钟，然后将上清液用于进一步的生化分析。
图示
图1.细胞裂解是指细胞的分解，通常用于分析特定的细胞组成，例如蛋白质，脂质，核酸，报告分子，细胞信号分子和其他小生物分子。根据所用的裂解液，全部或部分膜被溶解。ReadiUse 试剂需要少的动手时间。这种细菌细胞裂解缓冲液只需简单的5倍稀释即可。它广泛用于裂解细胞以定量细菌中的小生物分子，例如NAD（P）/ NAD（P）H。
参考文献
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Mechanics and Dynamics of Bacterial Cell Lysis
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Use of a modified bacterial ghost lysis system for the construction of an inactivated avian pathogenic Escherichia coli vaccine candidate
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