4%甲醛固定化溶液 ReadiUse

我们所有的ReadiUse 试剂都需要最少的手动操作时间。这种4％甲醛固定溶液是即用型。 它通常用于保存组织以进行常规组织学检查。

样本方案

注意：以下是我们推荐的协议，仅提供指南。 应根据您的具体需求进行修改。

1.进行甲醛固定：在室温下用4％甲醛孵育细胞10-30分钟。

注意：避免使用任何含甲醇的固定剂，因为甲醇会在固定过程中损害肌动蛋白。 优选的固定剂是不含甲醇的甲醛。

2.用PBS冲洗固定的细胞2-3次。

3.细胞准备好进行免疫组化/免疫荧光检测。

注意：将固定的细胞保存在4 ℃的PBS中。

参考文献

Rhinacanthin C Alleviates Amyloid-β Fibrils’ Toxicity on Neurons and Attenuates Neuroinflammation Triggered by LPS, Amyloid-β, and Interferon-γ in Glial Cells
Authors: Kai-An Chuang, Ming-Han Li, Ni-Hsuan Lin, Chih-Hsuan Chang, I Lu, I Pan, Tomoya Takahashi, Ming-Der Perng, Shu-Fang Wen
Journal: Oxidative Medicine and Cellular Longevity (2017)

Impact of siRNA targeting of β-catenin on differentiation of rat neural stem cells and gene expression of Ngn1 and BMP4 following in vitro hypoxic-ischemic brain damage
Authors: Xiaoying Zhang, Cuicui Zhu, Qiong Luo, Jv Dong, Lv Liu, Min Li, Hongtao Zhu, Xiangping Ma, Jun Wang
Journal: Molecular Medicine Reports (2016): 3595--3601

Application of interleukin-22 mediates protection in experimental acetaminophen-induced acute liver injury
Authors: Patrick Scheiermann, Malte Bachmann, Itamar Goren, Bernhard Zwissler, Josef Pfeilschifter, Heiko Mühl
Journal: The American journal of pathology (2013): 1107--1113

Synergistic activity of αvβ3 integrins and the elastin binding protein enhance cell-matrix interactions on bioactive hydrogel surfaces
Authors: Dhaval Patel, Susan E Vandromme, Michael E Reid, Lakeshia J Taite
Journal: Biomacromolecules (2012): 1420--1428