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派诺宁Y CAS 92-32-0

英文名称:Pyronin Y *CAS 92-32-0*
产品参数
Ex (nm)547Em (nm)566
分子量302.8溶剂DMSO
存储条件在零下15度以下保存, 避免光照
产品概述

产品基本信息

产品名称:派诺宁Y 

CAS:92-32-0

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:302.8

溶剂:DMSO

激发波长(nm):547

发射波长(nm):566

 

产品介绍

派诺宁Y是美国AAT Bioquest生产的荧光染料,派诺宁Y被广泛用于经SDS-PAGE电泳后结合甲基绿来选择性和特异性地染色核酸。在双染色溶液中,派诺宁Y将RNA染成红色,而甲基绿将DNA染成绿色。派诺宁Y *超纯级*的光谱相关参数为Ex=546nm / Em=549nm;其对应分子量大小为302.8MW。百萤生物是AAT Bioquest的中国代理商,为您提供优质的荧光染料。 

点击查看光谱

 

溶解方案(溶剂以说明书为准)

  0.1 mg 0.5 mg 1 mg 5 mg 10 mg
1 mM 330.251 µL 1.651 mL 3.303 mL 16.513 mL 33.025 mL
5 mM 66.05 µL 330.251 µL 660.502 µL 3.303 mL 6.605 mL
10 mM 33.025 µL 165.125 µL 330.251 µL 1.651 mL 3.303 mL

 

参考文献

A simple and high sensitive spectrophotometric method for ultra trace determination of ruthenium with its catalytic effect on the oxidation of pyronin B by periodate
Authors: Rezaei B, Majidi N.
Journal: Spectrochim Acta A Mol Biomol Spectrosc. (2006)

Methyl green-pyronin Y staining of nucleic acids: studies on the effects of staining time, dye composition and diffusion rates
Authors: Prento P, Lyon HO.
Journal: Biotech Histochem (2003): 27

Pyronin Y as a fluorescent stain for paraffin sections
Authors: Li B, Wu Y, Gao XM.
Journal: Histochem J (2002): 299

Functional heterogeneity within rhodamine123(lo) Hoechst33342(lo/sp) primitive hemopoietic stem cells revealed by pyronin Y
Authors: Huttmann A, Liu SL, Boyd AW, Li CL.
Journal: Exp Hematol (2001): 1109

Simultaneous three-color analysis of the surface phenotype and DNA-RNA quantitation using 7-amino-actinomycin D and pyronin Y
Authors: Toba K, Winton EF, Koike T, Shibata A.
Journal: J Immunol Methods (1995): 193

A methyl green-pyronin technique for demonstrating cell death in the murine tumour S180
Authors: Moffitt P.
Journal: Cell Biol Int (1994): 677

Effects of mounting media on fading of toluidine blue and pyronin G staining in epoxy sections
Authors: Schmolke C.
Journal: Biotech Histochem (1993): 132

Dye standards, Part II. 1: Pyronin Y (CI 45005)
Authors: European Committee for Clinical Laboratory Standards (ECCLS), Subcommittee on Reference Materials for Tissue Stains (SRMTS)
Journal: Histochem J (1992): 220

Simultaneous quantification of DNA and RNA in tissue sections. A comparative analysis of the methyl green-pyronin technique with the gallocyanin chromalum and Feulgen procedures using image cytometry
Authors: Schulte EK, Lyon HO, Hoyer PE.
Journal: Histochem J (1992): 305

1-naphthol-pyronin B as a novel substrate for silver intensification: application to light and electron microscopic immunocytochemistry of neuroendocrine systems
Authors: Toni R, Lechan RM.
Journal: J Histochem Cytochem (1990): 1209