细胞培养检测 Pluronic F-127 CAS 9003-11-6

产品基本信息

产品名称：Pluronic F-127 细胞培养检测

CAS：9003-11-6

储存条件：室内储存不要冷冻

保质期：12个月

产品物理化学光谱特性

分子量：~12500

溶剂：DMSO

产品介绍

Pluronic F-127是一种非离子表面活性剂，100％活性，对细胞相对，常用于染料AM酯，如Indo-1 AM，Fura-2 AM，Calcein AM，Fluo-3 AM，Fluo-4 AM，Quest Fluo-8 AM和Quest Rhod-4 AM等，以其水溶性。 Pluronic F-127也可用于分散其他亲脂性探针。应进行适当的控制以确保Pluronic F-127不会改变细胞的膜特性。为方便起见，我们还提供20％Pluronic F-127 DMSO溶液（Cat＃20052）和10％Pluronic F-127水溶液（Cat＃20053）。

操作说明

注意：以下是我们推荐的方案。该方案仅提供指南，实际应根据您的具体需求进行修改。

1.将1克Pluronic F-127溶于10毫升蒸馏水中，制成10％（w / v）原液，或2克Pluronic F-127在10毫升无水二甲基亚砜（DMSO）中制成20％（w / v）储备溶液。这些可能需要在40至50℃的温度下加热约30分钟。在室温下储存溶液。

注意：不要冷却或冷冻Pluronic F-127溶液，因为它可能会沉淀。如果观察到沉淀，可以通过加热至37℃使沉淀物溶解并在使用前涡旋。

2.将10％或20％Pluronic®F-127储备液（来自步骤1）稀释到细胞上样缓冲液中，如Hanks和20 mM Hepes缓冲液（HHBS），稀释度为1：1000至1：500，以达到0.02至0.04％的工作溶液。

3.然后将AM酯的DMSO储备溶液稀释到0.02至0.04％的工作溶液（来自步骤2）中，以获得1μM至10μM的最终AM酯浓度。

注意：Pluronic F-127的最终浓度通常保持在0.08％或以下。

4.将细胞在所需温度下孵育10分钟至1小时。

注意：通常，希望使用所需的最少量的AM酯来获得足够的荧光信号噪声水平。

5.标记后，在开始实验之前用HHBS或新鲜培养基洗涤细胞。

参考文献

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