细胞培养检测 Pluronic F-127 20% DMSO溶液 CAS 9003-11-6

产品基本信息

产品名称：Pluronic F-127 20% DMSO溶液

CAS：9003-11-6

储存条件：室内储存不要冷冻

保质期：6个月

产品物理化学光谱特性

分子量：~12500

溶剂：DMSO

注意：不要冷藏或冻结Pluronic®F-127溶液，因为它可能沉淀。如果观察到沉淀，则可在使用前加热至37°C并进行涡流处理。

产品介绍

Pluronic F-127是一种非离子表面活性剂，100％活性，对细胞相对，常用于染料AM酯，如Indo-1 AM，Fura-2 AM，Calcein AM，Fluo-3 AM，Fluo-4 AM，Quest Fluo-8 AM和Quest Rhod-4 AM等，以其水溶性。 Pluronic F-127也可用于分散其他亲脂性探针。应进行适当的控制以确保Pluronic F-127不会改变细胞的膜特性。为方便起见，我们还提供10％Pluronic F-127水溶液（Cat＃20053）和固体（Cat＃20050）。

操作说明

注意：以下是我们推荐的方案。该方案仅提供指南，应根据您的具体需求进行修改。

1.使用前立即将等体积的20％Pluronic F-127 DMSO溶液与AM酯DMSO储备溶液（1-5 mM）混合。

2.然后将AM酯和Pluronic F-127的溶液稀释到细胞上样缓冲液如Hanks和20mM Hepes缓冲液（HHBS）中，以达到1μM至10μM的最终AM酯浓度。

注意1：或者，可将稀释度为1：1000至1：500的20％Pluronic F-127储备液稀释至细胞上样缓冲液中，先沉淀0.02至0.04％的工作溶液，然后加入AM酯DMSO储备液实现最终AM酯浓度在1μM和10μM之间的溶液。

注意2：Pluronic F-127的最终浓度通常保持在0.08％或以下。

3.将细胞在所需温度下孵育10分钟至1小时。

注意：通常，希望使用所需的最少量的AM酯来获得足够的荧光信号噪声水平。

4.标记后，在开始实验之前用HHBS或新鲜培养基洗涤细胞。

参考文献

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