NTA 琥珀酰亚胺酯
英文名称：NTA succinimidyl ester
产品参数
| Ex (nm) | - | Em (nm) | - |
| 分子量 | 515.52 | 溶剂 | DMSO |
| 存储条件 | 在零下15度以下保存, 避免光照 |
产品概述
产品基本信息
产品名称：NTA 琥珀酰亚胺酯
储存条件：-15℃避光防潮
保质期：12个月
产品物理化学光谱特性
分子量：515.52
溶剂：DMSO
产品介绍
固定金属亲和色谱法（IMAC）是一种用于蛋白质纯化的流行方法，特别是对于与多组氨酸标签融合的重组蛋白质而言。 通过螯合配体固定在基质上的过渡金属离子与多组氨酸标签相互作用，有效地隔离了样品中的融合蛋白。标签-NTA缀合物用于检测多组氨酸标签的蛋白。 NTA琥珀酰亚胺酯是一种出色的构建基块，可轻松用于将NTA基团连接到具有氨基的生物底物或表面上。 通过附着在底物或表面上的NTA部分，可以通过Ni介导的His-Tag方法检测或固定带有六组氨酸延伸的基因表达蛋白（HIS标签）。
参考文献
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Authors: Lingg, Nico and Öhlknecht, Christoph and Fischer, Andreas and Mozgovicz, Markus and Scharl, Theresa and Oostenbrink, Chris and Jungbauer, Alois
Journal: Journal of chromatography. A (2020): 461649
Probing Protein-Protein Interactions with Label-Free Mass Spectrometry Quantification in Combination with Affinity Purification by Spin-Tip Affinity Columns.
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Journal: Analytical chemistry (2020): 3913-3922
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Journal: Analytical biochemistry (2019): 113347
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Journal: Protein expression and purification (2014): 1-7
Quantitative analysis of global phosphorylation changes with high-resolution tandem mass spectrometry and stable isotopic labeling.
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Journal: Methods (San Diego, Calif.) (2013): 251-9
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Journal: Langmuir : the ACS journal of surfaces and colloids (2012): 6885-92
