氯离子荧光探针MQAE CAS 162558-52-3

产品基本信息

产品名称：氯离子荧光探针MQAE

CAS：162558-52-3

储存条件：-15℃避光防潮

保质期：12个月

 

产品物理化学光谱特性

分子量：326.19

外观：黄色固体

溶剂：DMSO

激发波长（nm）：350

发射波长（nm）：460

 

产品介绍

氯离子荧光探针MQAE是美国AAT Bioquest生产的用于检测氯离子的荧光探针。氯化物指示剂，其荧光依赖于氯离子浓度。MQAE的荧光强度随着氯离子在细胞内的增加而成比例地减少。MQAE具有高水溶性和膜渗透能力，可以检测低于50 mM浓度的氯离子浓度而不受pH变化的影响。MQAE的检测灵敏度比SPQ更高，并且荧光效果也比SPQ要好一些，是一种目前最广泛应用的新型氯离子荧光探针。百萤生物是AAT Bioquest的中国代理商，为您提供优质的氯离子荧光探针MQAE。

点击查看光谱

 

操作步骤

1. 用Krebs-HEPES缓冲液洗涤细胞3次。
2. 将5 mM的Cl探针溶液加入细胞液中，并在37℃下培养1小时。
3. 用Krebs-HEPES缓冲液洗涤细胞5次。
4. 使用带有图像分析系统的激光共聚焦显微镜检测细胞的荧光强度。

注1：MQAE的工作液可以配制于Krebs-HEPES缓冲液中；Krebs-HEPES缓冲液配方（20mM HEPES，128mM NaCl，2.5mM KCl，2.7mM CaCl₂，1mM MgCl₂，16mM glucose，pH7.4）

注2：MQAE标记细胞时的浓度通常为5-10mM。以约800-1000万cell/ml的细胞密度，在含有5-10mM MQAE的Krebs-HEPES缓冲液中孵育30-60分钟。通常一个探针的装载体系为100ul即可。

 

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