死细胞核染料DiYO -1 (相当于 YOYO® 1) CAS 143413-85-8

DiYO -1在化学上等同于YOYO®-1（YOYO®是Invitrogen的商标）。DiYO -1是一种碳花青二聚体，具有类似于FITC的绿色荧光。它不具有细胞渗透性，可以很容易地与Cy5和罗丹明区别开来，作为核复染和死细胞指示剂。在不存在核酸的情况下它是非荧光的，并且在与DNA结合后会产生非常明亮的荧光信号。DiYO -1可在培养细胞和石蜡切片中进行强而有选择性的核染色。同时使用细胞可渗透的Nuclear Red LCS1染料和不渗透细胞的DiYO®-1进行标记，可用于评估细胞活力。DiYO -1和Nuclear Red 的消光系数都比结合DNA的碘化丙啶大。

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1.在磷酸盐缓冲液（PBS）中将DIYO-1染料稀释至2.4 nM，然后将200 µL稀释的DIYO-1溶液直接加到冲洗过的玻片上。
2.将样品与染色剂在室温下孵育至少10-20分钟，以获得佳染色效果。
3.用水冲洗载片，以未结合的染料和PBS。 从载玻片上多余的液体。
4.加入防淬灭剂，石蜡密封。
5.用配备了标准荧光素滤光片组的荧光显微镜观察样品。
6.可以在室温或4℃的光线下保存。

Correction: Photobleaching of YOYO-1 in super-resolution single DNA fluorescence imaging
Authors: J. R. Pyle
Journal: Beilstein J Nanotechnol (2018): 809-811

Ultrafast Transient Absorption Spectra of Photoexcited YOYO-1 molecules call for additional investigations of their fluorescence quenching mechanism
Authors: L. Wang
Journal: J Photochem Photobiol A Chem (2018): 411-419

Photobleaching of YOYO-1 in super-resolution single DNA fluorescence imaging
Authors: J. R. Pyle
Journal: Beilstein J Nanotechnol (2017): 2296-2306

Detection of amyloid-beta fibrils using the DNA-intercalating dye YOYO-1: Binding mode and fibril formation kinetics
Authors: D. J. Lindberg
Journal: Biochem Biophys Res Commun (2016): 313-8

Charging YOYO-1 on capillary wall for online DNA intercalation and integrating this approach with multiplex PCR and bare narrow capillary-hydrodynamic chromatography for online DNA analysis
Authors: H. Chen
Journal: Anal Chem (2015): 1518-22

Interference of ATP with the fluorescent probes YOYO-1 andYOYO-3 modifies the mechanical properties of intercalator-stained DNA confined in nanochannels
Authors: M. Roushan
Journal: Mikrochim Acta (2015): 1561-1565

Effect of YOYO-1 on the mechanical properties of DNA
Authors: B. Kundukad
Journal: Soft Matter (2014): 9721-8

Interaction of YOYO-1 with guanine-rich DNA
Authors: S. G. Datta
Journal: J Biomol Struct Dyn (2014): 1155-63

Feasibility of flow cytometry for measurements of Plasmodium falciparum parasite burden in studies in areas of malaria endemicity by use of bidimensional assessment of YOYO-1 and autofluorescence
Authors: J. J. Campo
Journal: J Clin Microbiol (2011): 968-74

Is photocleavage of DNA by YOYO-1 using a synchrotron radiation light source sequence dependent?
Authors: E. L. Gilroy
Journal: Eur Biophys J (2011): 1121-9