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ddATP [2',3'-Dideoxyadenosine-5'-triphosphate]

英文名称:ddATP [2',3'-Dideoxyadenosine-5'-triphosphate] *10 mM in ddH2O*
ddATP [2',3'-Dideoxyadenosine-5'-triphosphate]
价格 2683
产品规格
1 umole

产品货号
产品参数
Ex (nm)-Em (nm)-
分子量475.18溶剂Water
存储条件在零下15度以下保存, 避免光照
产品概述

产品基本信息

产品名称:ddTTP [2',3'-Dideoxythymidine-5'-triphosphate]

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:475.18

溶剂:水

 

产品介绍

Sanger测序,也称为链终止法,是一种基于DNA聚合酶选择性掺入链终止双脱氧核苷酸(ddNTPs)的DNA测序技术。它是由Frederick Sanger及其同事于1977年开发的。尽管新的NGS技术由于其较高的通量能力和较低的每份样品成本而在临床研究实验室中变得很普遍,但Sanger测序的准确度仍为99.99%,仍然是临床的“黄金标准”研究排序。 dd-ATP是执行Sanger测序的四个关键ddNTP组件之一。

 

参考文献

Denaturation fingerprinting: two related mutation detection methods especially advantageous for high G + C regions.
Authors: Liu, Q and Weinshenker, B G and Wingerchuk, D M and Sommer, S S
Journal: BioTechniques (1998): 140-7

Slow rate of phosphodiester bond formation accounts for the strong bias that Taq DNA polymerase shows against 2',3'-dideoxynucleotide terminators.
Authors: Brandis, J W and Edwards, S G and Johnson, K A
Journal: Biochemistry (1996): 2189-200

Recombinant human hepatitis B virus reverse transcriptase is active in the absence of the nucleocapsid or the viral replication origin, DR1.
Authors: Seifer, M and Standring, D N
Journal: Journal of virology (1993): 4513-20

The enzymatic termination of polydeoxynucleotides by 2',3'-dideoxyadenosine triphosphate.
Authors: Toji, L and Cohen, S S
Journal: Proceedings of the National Academy of Sciences of the United States of America (1969): 871-7