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Cy3 NHS酯 CAS 146368-16-3

英文名称:Cyanine 3 monosuccinimidyl ester [equivalent to Cy3® NHS ester]
产品参数
Ex (nm)555Em (nm)569
分子量829.03溶剂DMSO
存储条件在零下15度以下保存, 避免光照
产品概述

产品货期

现货

 

产品优势

1.水溶性好

2.消光系数高

3.摩尔吸收率大

4.高特异性和灵敏度

 

适用范围

用于标记肽、蛋白和核酸

 

产品介绍

AAT Bioquest生产的Cy3,NHS酯是胺反应性染料,用于标记氨基。常被用于生物分子标记,荧光成像及其他荧光生物分析。Cy3染料在与蛋白质结合后荧光增强。

点击查看光谱

实验图示

图1.将HeLa细胞与(Tubulin +)和(Tubulin-)小鼠抗微管蛋白分别孵育,然后与AAT的Cy3山羊抗小鼠IgG缀合物结合(红色,左);与Jackson 的Cy3缀合物结合(红色 ,右)。 细胞核用Hoechst 33342(Blue,Cat#17530)染色。

 

参考文献

CLASP-mediated competitive binding in protein condensates directs microtubule growth
Authors: Jia, Xuanyan and Lin, Leishu and Guo, Siqi and Zhou, Lulu and Jin, Gaowei and Dong, Jiayuan and Xiao, Jinman and Xie, Xingqiao and Li, Yiming and He, Sicong and others,
Journal: Nature Communications (2024): 6509
 
Competitive binding-mediated mesoscale protein-protein interactions direct microtubule growth
Authors: Wei, Zhiyi and Jia, Xuanyan and Lin, Leishu and Guo, Siqi and Zhou, Lulu and Jin, Gaowei and Dong, Jiayuan and Xiao, Jinman and Xie, Xingqiao and Li, Yiming and others,
Journal: (2024)
 
The disordered protein SERF promotes $\alpha$-Synuclein aggregation through liquid-liquid phase separation
Authors: Liu, He-Ning and Wang, Ting and Hu, Jin-Jian and Chen, Long and Shi, Xiangyan and Li, Yan-Mei and Luo, Shi-Zhong
Journal: Journal of Biological Chemistry (2024): 105667
 
Manganese regulation of COPII condensation controls circulating lipid homeostasis
Authors: Wang, Xiao and Huang, Runze and Wang, Yawei and Zhou, Wenjing and Hu, Yating and Yao, Yuanhang and Cheng, Kunlun and Li, Xin and Xu, Bolin and Zhang, Jie and others,
Journal: Nature Cell Biology (2023): 1--14
 
Temporal and spatial assembly of inner ear hair cell ankle link condensate through phase separation
Authors: Wang, Huang and Du, Haibo and Ren, Rui and Du, Tingting and Lin, Lin and Feng, Zhe and Zhao, Dange and Wei, Xiaoxi and Zhai, Xiaoyan and Wang, Hongyang and others,
Journal: Nature Communications (2023): 1657

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