Cell Meter 荧光法细胞电压检测试剂盒
英文名称：Cell Meter™ Fluorimetric Cellular Voltage Assay Kit
产品参数
| Ex (nm) | - | Em (nm) | - |
| 分子量 | - | 溶剂 | - |
| 存储条件 | - |
产品概述
几乎所有的质膜都具有跨膜电位，膜内侧相对于膜外侧通常为负电位。信号是通过在膜的某一点上打开或关闭离子通道，产生膜电位的局部变化而产生的。这种电场的变化可以迅速地受到膜中相邻或更远的离子通道的影响。这些离子通道由于电位的变化可以打开或关闭，从而再现信号。Cell Meter™荧光细胞电压检测试剂盒使用FRET对( VSB 405和VSR 555)来监测细胞膜电位的变化。亲脂性VSB 405主要位于脂质膜的外层，而VSR 555的定位对细胞膜电位敏感。在静息状态下，内层具有相对负电位，使得VSR 555主要位于靠近细胞膜外层，从而靠近蓝色荧光VSB405，导致有效的荧光从蓝色( VSB 405 )转移到红色( VSR 555 )。当细胞去极化时，VSR 555转移到细胞膜的内层，从而分离FRET对并破坏FRET。蓝色/红色荧光的比率与电池电压成正比。该方法可用于筛选调节离子通道的化合物。
适用仪器
荧光酶标仪
| Excitation： | 405 nm |
| Emission： | 460 nm and 580 nm |
| Cutoff： | 435 nm and 515 nm |
| 推荐孔板： | 黑色透明底板 |
| 读取模式： | 底读模式/分液处理 |
其他仪器
ArrayScan, FDSS, FLIPR, FlexStation, IN Cell Analyzer, ViewLux
试剂应用文献
Activation of the LINC00242/miR-141/FOXC1 axis underpins the development of gastric cancer
Authors: Zhong, Xiongdong and Yu, Xianchang and Wen, Xiaoyan and Chen, Lei and Gu, Ni
Journal: Cancer Cell International (2020): 1--11
Authors: Zhong, Xiongdong and Yu, Xianchang and Wen, Xiaoyan and Chen, Lei and Gu, Ni
Journal: Cancer Cell International (2020): 1--11
MicroRNA-1305 inhibits the stemness of LCSCs and tumorigenesis by repressing the UBE2T-dependent Akt-signaling pathway
Authors: Wei, Xiaoyong and You, Xiaolong and Zhang, Jianlong and Zhou, Cuncai
Journal: Molecular Therapy-Nucleic Acids (2019): 721--732
Authors: Wei, Xiaoyong and You, Xiaolong and Zhang, Jianlong and Zhou, Cuncai
Journal: Molecular Therapy-Nucleic Acids (2019): 721--732
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