Buccutite FOL，NHS酯

产品基本信息

产品名称：Buccutite FOL，NHS酯

储存条件：-15℃避光防潮

保质期：12个月

 

产品物理化学光谱特性

分子量：866.99

溶剂：DMSO

激发波长（nm）：N/A

发射波长（nm）：N/A

 

产品介绍

我们的Buccutite 交联技术提供了方便有效的交联方法，可以将两种生物分子连接在一起，具有很高的共轭产率。我们的方法使用一对交联剂：Buccutite MTA和Buccutite FOL。将MTA添加到一个分子中，而将FOL添加到另一个分子中。通过混合Molecule-1-Buccutite MTA和Molecule-2-Buccutite FOL引发交联反应。该交联反应在极端温和的中性条件下进行，无需任何催化剂。它稳定而。我们的许多客户要求我们提供独立的Buccutite MTA和Buccutite FOL试剂，以扩展Buccutite 交联技术的应用。蛋白质 - 蛋白质缀合通常用双功能接头如SMCC进行。SMCC的一端（通过NHS酯）与氨基酸赖氨酸和N-末端中发现的胺（-NH2）反应，另一端（通过马来酰亚胺）与氨基酸中发现的硫醇基团（-SH）反应。半胱氨酸。然而，SMCC修饰的蛋白质极不稳定并且通常是自身反应性的，因为蛋白质通常含有胺和硫醇基团，其引起显着量的均聚交联。此外，量化蛋白质上马来酰亚胺基团的数量是相当困难和繁琐的。百萤生物是AAT Bioquest的中国代理商，为您提供优质的Buccutite FOL，NHS酯。

 

参考文献

Identification of pyridinoline, a collagen crosslink, as a novel intrinsic ligand for the receptor for advanced glycation end-products (RAGE)
Authors: Y. Murakami
Journal: Biosci Biotechnol Biochem (2018): 1508-1514

Structure-based non-canonical amino acid design to covalently crosslink an antibody-antigen complex
Authors: J. Xu
Journal: J Struct Biol (2014): 215-22

Comparison of 1-Ethyl-3-(3-Dimethylaminopropyl) Carbodiimide Based Strategies to Crosslink Antibodies on Amine-Functionalized Platforms for Immunodiagnostic Applications
Authors: S. K. Vashist
Journal: Diagnostics (Basel) (2012): 23-33

[Promotion of the activity of Akt in CD8+ T cells by the crosslink of 3H3, an agonist antibody of 4-1BB]
Authors: Z. Yang
Journal: Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi (2007): 223-5

Requirement for PCNA and RPA in interstrand crosslink-induced DNA synthesis
Authors: L. Li
Journal: Nucleic Acids Res (2000): 1424-7

Does properdin crosslink the cellular and the humoral immune response?
Authors: W. J. Schwaeble
Journal: Immunol Today (1999): 17-21

Evaluation of an immunoassay method for the determination of urinary collagen crosslink excretion
Authors: K. Hata
Journal: Ann Clin Biochem (1994): 374-5

Antibodies against DNA-psoralen crosslink recognize unique conformation
Authors: R. Hasan
Journal: Biochim Biophys Acta (1991): 509-13

Persistence of early crosslink-dependent signal transduction events in human basophils after desensitization
Authors: J. Warner
Journal: Immunol Lett (1988): 129-37

An enzyme-linked immunosorbent assay to quantitate the elastin crosslink desmosine in tissue and urine samples
Authors: Z. Gunja-Smith
Journal: Anal Biochem (1985): 258-64

 

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