生物素-X IDA

产品基本信息

产品名称：生物素-X IDA

储存条件：-15℃避光防潮

保质期：12个月

产品物理化学光谱特性

分子量：515.63

外观：固体

溶剂：水

产品介绍

固定化金属亲和层析 (IMAC) 是一种常用的蛋白质纯化方法，特别是对于融合到多组氨酸标签的重组蛋白质。通过螯合配体固定在基质上的过渡金属离子与多组氨酸标签相互作用，有效地从样品中隔离融合蛋白。次氮基三乙酸 (NTA) 和亚氨基二乙酸 (IDA) 是市售树脂中常用的两种此类配体。 AAT Bioquest 提供各种 NTA 构建模块，用于开发基于 NTA 的纯化和检测。 IDA 对 NTA 是免费的。三齿 IDA 配体需要比四齿 NTA 更低的咪唑浓度来洗脱蛋白质。 IDA 是一种较小的分子，可以以更高的密度与基质偶联，从而导致更高的金属负载能力。生物素 IDA 是一种双功能试剂，可用于检测固定的组氨酸标签蛋白。生物素 IDA 可以从 pH 4.8 的组氨酸标签蛋白中去除，从而允许使用另一种探针重新分析印迹。百萤生物是AAT Bioquest的中国代理商，为您提供优质的生物素-X IDA。

参考文献

Synchronized purification and immobilization of his-tagged β-glucosidase via Fe3O4/PMG core/shell magnetic nanoparticles.
Authors: Zhou, Yang and Yuan, Shaofei and Liu, Qian and Yan, Dandan and Wang, Yun and Gao, Li and Han, Juan and Shi, Haifeng
Journal: Scientific reports (2017): 41741

Grafting iminodiacetic acid on silica nanoparticles for facilitated refolding of like-charged protein and its metal-chelate affinity purification.
Authors: Liu, Hu and Dong, Xiaoyan and Sun, Yan
Journal: Journal of chromatography. A (2016): 277-83

Uniform magnetic core/shell microspheres functionalized with Ni2+-iminodiacetic acid for one step purification and immobilization of his-tagged enzymes.
Authors: Zhang, Yuting and Yang, Yongkun and Ma, Wanfu and Guo, Jia and Lin, Yao and Wang, Changchun
Journal: ACS applied materials & interfaces (2013): 2626-33

Dual affinity method for plasmid DNA purification in aqueous two-phase systems.
Authors: Barbosa, H S C and Hine, A V and Brocchini, S and Slater, N K H and Marcos, J C
Journal: Journal of chromatography. A (2010): 1429-36

Evaluation of IDA-PEVA hollow fiber membrane metal ion affinity chromatography for purification of a histidine-tagged human proinsulin.
Authors: de Aquino, Luciana Cristina Lins and de Sousa, Heloisa Ribeiro Tunes and Miranda, Everson Alves and Vilela, Luciano and Bueno, Sônia Maria Alves
Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (2006): 68-76

Overproduction of Thermus sp. Strain T2 beta-galactosidase in Escherichia coli and preparation by using tailor-made metal chelate supports.
Authors: Pessela, Benevides C C and Vian, Alejandro and Mateo, César and Fernández-Lafuente, Roberto and García, José L and Guisán, José M and Carrascosa, Alfonso V
Journal: Applied and environmental microbiology (2003): 1967-72

Evidence for phosphorylation of serine 753 in CFTR using a novel metal-ion affinity resin and matrix-assisted laser desorption mass spectrometry.
Authors: Neville, D C and Rozanas, C R and Price, E M and Gruis, D B and Verkman, A S and Townsend, R R
Journal: Protein science : a publication of the Protein Society (1997): 2436-45

Cell chromatography: separation of different microbial cells using IMAC supermacroporous monolithic columns.
Authors: Dainiak, Maria B and Plieva, Fatima M and Galaev, Igor Yu and Hatti-Kaul, Rajni and Mattiasson, Bo
Journal: Biotechnology progress: 644-9

One-step purification, covalent immobilization, and additional stabilization of a thermophilic poly-His-tagged beta-galactosidase from Thermus sp. strain T2 by using novel heterofunctional chelate-epoxy Sepabeads.
Authors: Pessela, Benevides C C and Mateo, Cesar and Carrascosa, Alfonso V and Vian, Alejandro and García, José L and Rivas, German and Alfonso, Carlos and Guisan, José M and Fernández-Lafuente, Roberto
Journal: Biomacromolecules: 107-13