生物胞素 C2 马来酰亚胺

产品基本信息

产品名称：生物胞素 C2 马来酰亚胺

储存条件：-15℃避光防潮

保质期：12个月

产品物理化学光谱特性

分子量：494.61

溶剂：DMSO

激发波长（nm）：N/A

发射波长（nm）：N/A

产品介绍

生物胞素 C2 马来酰亚胺是美国AAT Bioquest生产的生物素类荧光探针。生物胞素马来酰亚胺易与生物大分子硫羟基部分反应，形成硫醚偶联物，它十分稳定。生物胞素马来酰亚胺需要温和地结合条件。例如，在PH5.5通常是修饰半胱氨酸残基的佳PH， 并且无论什么时候，尽量减少暴露在空气中溶液反应，尽可能避免硫羟化底物在空气中氧化。大多偶联物在室温下形成。然而，升高或者降低温度都可能需要特定的标记反应。生物胞素必须置于无外来硫醇的缓冲液中（6-巯基乙醇、二硫苏糖醇和巯基乙胺）反应。蛋白质或者多肽通过与不含有硫醇基团（SH）的试剂进行硫羟化反应形成生物胞素。百萤生物是AAT Bioquest的中国代理商，为您提供优质的生物胞素 C2 马来酰亚胺。 

点击查看实验方案

参考文献

The anterograde and retrograde axonal transport of biotinylated dextran amine and biocytin in the nervous system of teleosts
Authors: Xue HG, Yang CY, Ito H.
Journal: Brain Res Brain Res Protoc (2004): 106

Biocytin and biotin uptake into NB2a neuroblastoma and C6 astrocytoma cells
Authors: Baur B, Suormala T, Baumgartner ER.
Journal: Brain Res (2002): 111

Correlative fluorescence and electron microscopy of biocytin-filled neurons with a preservation of the postsynaptic ultrastructure
Authors: Morozov Y, Khalilov I, Ben-Ari Y, Represa A.
Journal: J Neurosci Methods (2002): 81

A new quantitative analytical method of serum biotinidase activity using biocytin as a substrate and its clinical significance in Japan
Authors: Kumasaka K, Muratsugu M, Fukui T, Kimura M, Takagi Y, Hashizume N.
Journal: Clin Chim Acta (2001): 71

Biotin and biocytin uptake into cultured primary calf brain microvessel endothelial cells of the blood-brain barrier
Authors: Baur B, Baumgartner ER.
Journal: Brain Res (2000): 348

In vitro biocytin injection into perinatal mouse brain: a method for tract tracing in developing tissue
Authors: Chang SL, LoTurco JJ, Nisenbaum LK.
Journal: J Neurosci Methods (2000): 1

A method of combining biocytin tract-tracing with avidin-biotin-peroxidase complex immunocytochemistry for pre-embedding electron microscopic labeling in neonatal tissue
Authors: Erisir A, Aoki C.
Journal: J Neurosci Methods (1998): 189

A radioiodinated biocytin derivative for in-vivo applications
Authors: Nakamoto Y, Sakahara H, Saga T, Yao Z, Zhang M, Sato N, Zhao S, Arano Y, Ono M, Saji H, Konishi J.
Journal: Nucl Med Commun (1998): 963

A rapid method for combined laser scanning confocal microscopic and electron microscopic visualization of biocytin or neurobiotin-labeled neurons
Authors: Sun XJ, Tolbert LP, Hildebrand JG, Meinertzhagen IA.
Journal: J Histochem Cytochem (1998): 263

Biotinylated dextran amine and biocytin hydrochloride are useful tracers for the study of retinal projections in the frog
Authors: Scalia F, Galoyan SM, Eisner S, Harris E, Su W.
Journal: J Neurosci Methods (1997): 167