ATTO 647酸
英文名称：ATTO 647 acid
产品参数
| Ex (nm) | 646 | Em (nm) | 666 |
| 分子量 | 706.81 | 溶剂 | DMSO |
| 存储条件 | 在零下15度以下保存, 避免光照 |
产品概述
产品货期
咨询
产品优势
1.高量子产率和光稳定性：为敏感应用提供强大、稳定的荧光
2.亲水性：增强在水性环境中的溶解度
3.两性离子： 最大限度地减少与生物表面的非特异性结合，减少活细胞成像和超分辨率显微镜中的背景噪音
适用范围
标记肽、抗体、蛋白质、寡核苷酸
产品介绍
AAT Bioquest生产的ATTO647 酸是一种罗丹明衍生荧光染料，针对红色光谱区域内的应用进行了优化，具有与 Cy5 类似的光谱特性。该染料具有高度亲水性，最大激发波长范围为 615-660 nm。作为一种两性离子分子，ATTO 647 带有净中性电荷。该染料在生理 pH 条件下甚至pH值高达 8 的缓冲液中稳定性好。ATTO 647 是单分子检测和高分辨率显镜技术（包括 PALM、dSTORM 和 STED 显微镜）的理想选择。它还与流式细胞术（FACS）、荧光位杂交（FISH）、FRET 以及各种其他生物测定兼容。
ATTO 647 酸是一种非反应性化合物，可在ATTO 647 缀合物的研究中用作参考标准。适合用于共焦显微镜、免疫细胞化学 (ICC)、高通量筛选 (HCS)、流式细胞术和活细胞成像应用中的对照。用于合成活化酯和STP，使用碳二亚胺（例如EDAC）在水溶液中与肼、羟胺或胺偶联。与含氨基的分子缀合，例如蛋白质、抗体、胺修饰的寡核苷酸和肽。
试剂应用文献
Single-molecule enzymatic reaction dynamics and mechanisms of GPX3 and TRXh9 from Arabidopsis thaliana
Authors: Kuang, Yanmin and Guo, Xing and Guo, Aiyu and Ran, Xia and He, Yulu and Zhang, Yu and Guo, Lijun
Journal: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy (2020): 118778
Authors: Kuang, Yanmin and Guo, Xing and Guo, Aiyu and Ran, Xia and He, Yulu and Zhang, Yu and Guo, Lijun
Journal: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy (2020): 118778
A novel nanocomposite based on fluorescent turn-on gold nanostars for near-infrared photothermal therapy and self-theranostic caspase-3 imaging of glioblastoma tumor cell
Authors: Wang, J., Zhou, Z., Zhang, F., Xu, H., Chen, W., Jiang, T.
Journal: Colloids Surf B Biointerfaces (2018): 303-311
Authors: Wang, J., Zhou, Z., Zhang, F., Xu, H., Chen, W., Jiang, T.
Journal: Colloids Surf B Biointerfaces (2018): 303-311
Cell-permeable organic fluorescent probes for live-cell long-term super-resolution imaging reveal lysosome-mitochondrion interactions
Authors: Han, Y., Li, M., Qiu, F., Zhang, M., Zhang, Y. H.
Journal: Nat Commun (2017): 1307
Authors: Han, Y., Li, M., Qiu, F., Zhang, M., Zhang, Y. H.
Journal: Nat Commun (2017): 1307
Field-Controlled Charge Separation in a Conductive Matrix at the Single-Molecule Level: Toward Controlling Single-Molecule Fluorescence Intermittency
Authors: Kennes, K., Dedecker, P., Hutchison, J. A., Fron, E., Uji, I. H., Hofkens, J., Van der Auweraer, M.
Journal: ACS Omega (2016): 1383-1392
Authors: Kennes, K., Dedecker, P., Hutchison, J. A., Fron, E., Uji, I. H., Hofkens, J., Van der Auweraer, M.
Journal: ACS Omega (2016): 1383-1392
Determination of equilibrium and rate constants for complex formation by fluorescence correlation spectroscopy supplemented by dynamic light scattering and Taylor dispersion analysis
Authors: Zhang, X., Poniewierski, A., Jelinska, A., Zagozdzon, A., Wisniewska, A., Hou, S., Holyst, R.
Journal: Soft Matter (2016): 8186-8194
Authors: Zhang, X., Poniewierski, A., Jelinska, A., Zagozdzon, A., Wisniewska, A., Hou, S., Holyst, R.
Journal: Soft Matter (2016): 8186-8194
