碱性磷酸酶[CIAP]

产品基本信息

产品名称：碱性磷酸酶[CIAP]

储存条件：-15℃避光防潮

保质期：6个月

产品物理化学光谱特性

外观：固体

溶剂：水

产品介绍

牛小肠碱性磷酸酶（CIP或CIAP）非特异性地催化DNA和RNA磷酸单酯5'和3'末端的去磷酸化。 CIAP还水解核糖以及脱氧核糖核苷三磷酸（NTP和dNTP）。 CIAP可用于许多分子生物学应用，例如去除DNA和RNA的磷酸化末端，以便随后用于探针的克隆或末端标记。 在克隆中，去磷酸化阻止线性化质粒DNA的重新连接。CIAP还可以用于降解PCR反应中未掺入的dNTP，以制备用于DNA测序或SNP分析的模板。 CIAP结合物已与多种有色或荧光AP底物结合广泛用于免疫学分析和测试中。 AAT Bioquest提供了大量的荧光AP底物，用于开发基于AP的ELISA分析和测试。

参考文献

CXCL12-CXCR4 Interplay Facilitates Palatal Osteogenesis in Mice.
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Effects of intestinal alkaline phosphatase on intestinal barrier function in a cecal ligation and puncture (CLP)-induced mouse model for sepsis.
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Journal: Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society (2020): e13754

Loss of tissue-nonspecific alkaline phosphatase (TNAP) enzyme activity in cerebral microvessels is coupled to persistent neuroinflammation and behavioral deficits in late sepsis.
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Journal: Brain, behavior, and immunity (2020): 115-131

Considering the Effect of Rosa damascena Mill. Essential Oil on Oxidative Stress and COX-2 Gene Expression in the Liver of Septic Rats.
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Hepatoprotective Effect of Echinochrome Pigment in Septic Rats.
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Multiple layers of regulation determine the cellular levels of the Pup ligase PafA in Mycobacterium smegmatis.
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Protective role of thymoquinone in sepsis-induced liver injury in BALB/c mice.
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Hepatoprotective Effect of Essential Oils from Hyptis crenata in Sepsis-Induced Liver Dysfunction.
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Overexpression of homeodomain-interacting protein kinase 2 (HIPK2) attenuates sepsis-mediated liver injury by restoring autophagy.
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Insulin Modulates Liver Function in a Type I Diabetes Rat Model.
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