蛋白酶体20S荧光底物(Ac-KQL)2R110

产品基本信息

产品名称：蛋白酶体20S荧光底物(Ac-KQL)2R110

储存条件：-15℃避光防潮

保质期：12个月

产品物理化学光谱特性

分子量：1153.33

溶剂：DMSO

激发波长（nm）：498

发射波长（nm）：520

产品介绍

蛋白酶体20S荧光底物(Ac-KQL)2R110是美国AAT Bioquest生产的荧光底物，非荧光性R110底物产生明亮的绿色荧光R110产物（Ex/Em = 494/521 nm），很容易被带有FITC滤光器所检测。一般来说，R110底物比AMC-, AFC- or 4-硝基苯胺的底物更敏感。R110底物用来检测蛋白酶体中蛋白酶的活性。蛋白酶体通用的形式就是26s蛋白酶体，它包含一个20S的核心结构颗粒和两个19S调节调节基团。所有20S的核心结构颗粒包含四对七聚物环状结构组成两种不同的亚基，alpha亚基是结构亚基，beta亚基主要是催化亚基。在两个环状结构外每个含有七个alpha亚基主要是为了调整颗粒的停靠。Alpha亚基N端结构是可以阻挡未调节底物进入内腔的门户。内腔两个环分别包含七个beta亚基包含酶位点来执行蛋白水解反应。AAT Bioquest提供一系列R110底物用于检测蛋白酶体中不同底物的蛋白酶活性，i.e., (i) sub-sites: _1c, Z-LLE-R110; _2c, Ac-KQL-R110; _5c, Ac-WLA-R110; _1i, Ac-PAL-R110; _2i, Ac-KQL-R110; _5c, Ac-WLA-R110和Suc-LLVY-R110;和_5i, Ac-ANW-R110。蛋白酶的活性能通过R110释放而测定（Ex/Em = 490/520 nm）。百萤生物是AAT Bioquest的中国代理商，为您提供优质的蛋白酶体20S荧光底物(Ac-KQL)2R110。 

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参考文献

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