AA-dUTP [氨基烯丙基 dUTP 钠盐] *4 mM TE 缓冲液 (pH 7.5)* *CAS 936327-10-5*

产品基本信息

产品名称：AA-dUTP [氨基烯丙基 dUTP 钠盐] *4 mM TE 缓冲液 (pH 7.5)* *CAS 936327-10-5*

CAS：936327-10-5

储存条件：-15℃避光防潮

保质期：12个月

产品物理化学光谱特性

分子量：589.17

溶剂：水

激发波长(nm)：N/A

发射波长(nm)：N/A

产品介绍

氨基烯丙基-DUTP钠盐 4mM AA-dUTP是美国AAT Bioquest生产的荧光染料，胺修饰的脱氧尿苷5'-三磷酸酯（如氨基烯丙基-dUTP）可用于通过常规酶掺入方法（如反转录，缺口翻译，随机引物标记或PCR）生产含胺的DNA。氨基烯丙基dUTP可以通过常规酶结合技术轻松地掺入DNA中。然后可以使用任何胺反应性荧光染料，生物素和其他胺反应性试剂对所得的胺修饰的核酸进行标记。与通常具有较高立体抑制能力的标签标记的尿苷三磷酸酯相比，氨基烯丙基修饰的核苷酸可以掺入到极高且一致的水平。胺修饰的核酸与过量的胺反应性试剂的随后反应，选择何种标记试剂，均能实现相应较高的一致标记效率。这种两步标记方法还了优化酶促反应以适应不同染料修饰核苷酸的需要，该修饰可能以非常不同的速率掺入。这种标记方法广泛用于FISH探针和基于微阵列的实验。百萤生物是AAT Bioquest的中国代理商，为您提供优质的氨基烯丙基-DUTP钠盐 4mM AA-dUTP 。

参考文献

A visual DNA chip for simultaneous detection of hepatitis B virus, hepatitis C virus and human immunodeficiency virus type-1
Authors: Wen JK, Zhang XE, Cheng Z, Liu H, Zhou YF, Zhang ZP, Yang JH, Deng JY.
Journal: Biosens Bioelectron (2004): 685

Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling
Authors: Cox WG, Singer VL.
Journal: Biotechniques (2004): 114

Aminomodified nucleobases: functionalized nucleoside triphosphates applicable for SELEX
Authors: Schoetzau T, Langner J, Moyroud E, Roehl I, Vonhoff S, Klussmann S.
Journal: Bioconjug Chem (2003): 919

Simple method for preparation of fluor/hapten-labeled dUTP
Authors: Nimmakayalu M, Henegariu O, Ward DC, Bray-Ward P.
Journal: Biotechniques (2000): 518

Topology of yeast RNA polymerase II subunits in transcription elongation complexes studied by photoaffinity cross-linking
Authors: Wooddell CI, Burgess RR.
Journal: Biochemistry (2000): 13405

Quantitative analysis of polymerase chain reaction products using biotinylated dUTP incorporation
Authors: Duplaa C, Couffinhal T, Labat L, Moreau C, Lamaziere JM, Bonnet J.
Journal: Anal Biochem (1993): 229

A non-radioisotopic reverse transcriptase assay using biotin-11-deoxyuridinetriphosphate on primer-immobilized microtiter plates
Authors: Urabe T, Sano K, Tanno M, Mizoguchi J, Otani M, Lee MH, Takasaki T, Kusakabe H, Imagawa DT, Nakai M.
Journal: J Virol Methods (1992): 145

Affinity isolation of active murine erythroleukemia cell chromatin: uniform distribution of ubiquitinated histone H2A between active and inactive fractions
Authors: Dawson BA, Herman T, Haas AL, Lough J.
Journal: J Cell Biochem (1991): 166

Non-radioactive labeling and detection of nucleic acids. IV. Synthesis and properties of digoxigenin-modified 2'-deoxyuridine-5'-triphosphates and a photoactivatable analog of digoxigenin (photodigoxigenin)
Authors: Muhlegger K, Huber E, von der Eltz H, Ruger R, Kessler C.
Journal: Biol Chem Hoppe Seyler (1990): 953