HIS Lite OG488-Tris NTA-Ni 复合物
英文名称：HIS Lite™ OG488-Tris NTA-Ni Complex
产品参数
| Ex (nm) | 498 | Em (nm) | 526 |
| 分子量 | 1838.08 | 溶剂 | Water |
| 存储条件 | 在零下15度以下保存, 避免光照 |
产品概述
产品基本信息
产品名称：HIS Lite OG488-Tris NTA-Ni复合物
储存条件：-15℃避光防潮
保质期：24个月
备注：该产品荧光受pH影响，购买前请详细咨询
产品物理化学光谱特性
分子量：1838.08
溶剂：水
激发波长(nm)：N/A
发射波长(nm)：N/A
产品介绍
HIS Lite OG488-Tris NTA-Ni复合物是美国AAT Bioquest生产的荧光染料，荧光tris-NTA化合物为聚组氨酸标签的蛋白的位点特异性和稳定的非共价荧光标记提供了有效的方法。与常规单-NTA的瞬时结合相反，与tris-NTA结合的荧光团的多价相互作用与带有多组氨酸标签的蛋白形成了更加稳定的复合物。tris-NTA化合物对累积的组氨酸具有很高的选择性，可以选择性标记细胞裂解液和活细胞表面上的蛋白质。荧光tris-NTA偶联物可用于分析溶液中和表面上的三元蛋白质复合物。过渡金属离子（例如，Ni离子）介导的多组氨酸标记的蛋白质与荧光tris-NTA偶联物的络合提供了灵敏的报告基因，用于实时检测和监测蛋白质-蛋白质相互作用。据报道，这种OG488-tris-NTA化合物是一种灵敏的荧光探针，可检测细胞，溶液和固体表面中多组氨酸标记的蛋白质。百萤生物是AAT Bioquest的中国代理商，为您提供优质的HIS Lite OG488-Tris NTA-Ni复合物。
试剂应用文献
Escherichia coli tRNA 2-selenouridine synthase SelU selects its prenyl substrate to accomplish its enzymatic function
Authors: Szczupak, Patrycja and Radzikowska-Cieciura, Ewa and Kulik, Katarzyna and Madaj, Rafa{\l} and Sierant, Ma{\l}gorzata and Krakowiak, Agnieszka and Nawrot, Barbara
Journal: Bioorganic Chemistry (2022): 105739
Authors: Szczupak, Patrycja and Radzikowska-Cieciura, Ewa and Kulik, Katarzyna and Madaj, Rafa{\l} and Sierant, Ma{\l}gorzata and Krakowiak, Agnieszka and Nawrot, Barbara
Journal: Bioorganic Chemistry (2022): 105739
Selective binding, magnetic separation and purification of histidine-tagged protein using biopolymer magnetic core-shell nanoparticles
Authors: Zhou, Y., Yan, D., Yuan, S., Chen, Y., Fletcher, E. E., Shi, H., Han, B.
Journal: Protein Expr Purif (2018): 5-11
Authors: Zhou, Y., Yan, D., Yuan, S., Chen, Y., Fletcher, E. E., Shi, H., Han, B.
Journal: Protein Expr Purif (2018): 5-11
Quantification of Histidine-Rich Protein 3 of Plasmodium falciparum
Authors: Palani, B.
Journal: Monoclon Antib Immunodiagn Immunother (2018): 87-90
Authors: Palani, B.
Journal: Monoclon Antib Immunodiagn Immunother (2018): 87-90
A novel reverse micellar purification strategy for histidine tagged human interferon gamma (hIFN-gamma) protein from Pichia pastoris
Authors: Prabhu, A. A., Purkayastha, A., M and al, B., Kumar, J. P., M and al, B. B., Veeranki, V. D.
Journal: Int J Biol Macromol (2018): 2512-2524
Authors: Prabhu, A. A., Purkayastha, A., M and al, B., Kumar, J. P., M and al, B. B., Veeranki, V. D.
Journal: Int J Biol Macromol (2018): 2512-2524
Label-Free Direct Electrical Detection of a Histidine-Rich Protein with Sub-Femtomolar Sensitivity using an Organic Field-Effect Transistor
Authors: Minamiki, T., Sasaki, Y., Tokito, S., Minami, T.
Journal: ChemistryOpen (2017): 472-475
Authors: Minamiki, T., Sasaki, Y., Tokito, S., Minami, T.
Journal: ChemistryOpen (2017): 472-475
Controlling Protein Surface Orientation by Strategic Placement of Oligo-Histidine Tags
Authors: Wasserberg, D., Cabanas-Danes, J., Prangsma, J., O'Mahony, S., Cazade, P. A., Tromp, E., Blum, C., Thompson, D., Huskens, J., Subramaniam, V., Jonkheijm, P.
Journal: ACS Nano (2017): 9068-9083
Authors: Wasserberg, D., Cabanas-Danes, J., Prangsma, J., O'Mahony, S., Cazade, P. A., Tromp, E., Blum, C., Thompson, D., Huskens, J., Subramaniam, V., Jonkheijm, P.
Journal: ACS Nano (2017): 9068-9083
Nonfouling NTA-PEG-Based TEM Grid Coatings for Selective Capture of Histidine-Tagged Protein Targets from Cell Lysates
Authors: Benjamin, C. J., Wright, K. J., Hyun, S. H., Krynski, K., Yu, G., Bajaj, R., Guo, F., Stauffacher, C. V., Jiang, W., Thompson, D. H.
Journal: Langmuir (2016): 551-9
Authors: Benjamin, C. J., Wright, K. J., Hyun, S. H., Krynski, K., Yu, G., Bajaj, R., Guo, F., Stauffacher, C. V., Jiang, W., Thompson, D. H.
Journal: Langmuir (2016): 551-9
Ni(II)NTA AuNPs as a low-resource malarial diagnostic platform for the rapid colorimetric detection of Plasmodium falciparum Histidine-Rich Protein-2
Authors: Gulka, C. P., Swartz, J. D., Wright, D. W.
Journal: Talanta (2015): 94-101
Authors: Gulka, C. P., Swartz, J. D., Wright, D. W.
Journal: Talanta (2015): 94-101
Coffee rings as low-resource diagnostics: detection of the malaria biomarker Plasmodium falciparum histidine-rich protein-II using a surface-coupled ring of Ni(II)NTA gold-plated polystyrene particles
Authors: Gulka, C. P., Swartz, J. D., Trantum, J. R., Davis, K. M., Peak, C. M., Denton, A. J., Haselton, F. R., Wright, D. W.
Journal: ACS Appl Mater Interfaces (2014): 6257-63
Authors: Gulka, C. P., Swartz, J. D., Trantum, J. R., Davis, K. M., Peak, C. M., Denton, A. J., Haselton, F. R., Wright, D. W.
Journal: ACS Appl Mater Interfaces (2014): 6257-63
High-affinity gold nanoparticle pin to label and localize histidine-tagged protein in macromolecular assemblies
Authors: Anthony, K. C., You, C., Piehler, J., Pomeranz Krummel, D. A.
Journal: Structure (2014): 628-35
Authors: Anthony, K. C., You, C., Piehler, J., Pomeranz Krummel, D. A.
Journal: Structure (2014): 628-35
