iFluor 860-标记链霉亲和素偶联物
Ex (nm) | 853 | Em (nm) | 878 |
分子量 | ~52000 | 溶剂 | Water |
存储条件 | 在零下15度以下保存, 避免光照 |
产品基本信息
产品名称:iFluor 860-标记链霉亲和素偶联物
储存条件:-15℃避光防潮
保质期:24个月
产品物理化学光谱特性
分子量:~52000
溶剂:DMSO
激发波长(nm):853
发射波长(nm):878
产品介绍
链霉亲和素偶联物 iFluor 860-标记链霉亲和素偶联物是美国AAT Bioquest生产的链霉亲和素,AAT 生物科技公司的 iFluor 染料在标记蛋白尤其是标记抗体是佳的。这些染料是非常明亮的并且耐光性好,在标记蛋白质时具有小的荧光淬灭。他们可以很好的被现有的主要的激光荧光激发仪器(如350、405、488、555和633nm)激发。链霉亲和素轭合物是与生物素共轭广泛应用于特殊检测的多种蛋白质,蛋白质片段、核酸和其他来自链霉亲和素的分子,与生物素具有很高的亲和力。众多的供应商提供各种各样的互补性生物素衍生物试剂。这一iFluor 860 -链霉亲和素是由包括链霉亲和素(如生物素结合的蛋白)和iFluor 840(如荧光标记)通过共价链接生成。用于与生物素结合的抗体时它常用作第二步间接免疫荧光染色试剂。它是以生物素链霉亲和素为基础的一个非常有价值的生物测定和实验工具。百萤生物是AAT Bioquest的中国代理商,为您提供优质的iFluor 860-标记链霉亲和素偶联物。
图示
图1. AAT Bioquest的明亮且耐光的iFluor 染料覆盖200至900 nm的整个UV,Vis和IR光谱。它们可以被荧光仪器激发的主要激光线(例如350、405、488、555、633、647和860 nm)。iFluor 染料标记的链霉亲和素偶联物与生物素偶联物一起广泛用于特异性检测多种蛋白质,蛋白质基序,核酸和其他分子,因为链霉亲和素对生物素具有非常高的结合亲和力。
参考文献
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Streptavidin cooperative allosterism upon binding biotin observed by differential changes in intrinsic fluorescence
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Design and Fabrication of Streptavidin-Functionalized, Fluorescently Labeled Polymeric Nanocarriers
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Optimized Streptavidin for Fluorescent Labeling of Biotinylated Targets
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