iFluor™ 488 TCO

产品基本信息

产品名称：iFluor™ 488 TCO

储存条件：-15℃避光防潮

保质期：12个月

产品物理化学光谱特性

分子量：856.79

外观：固体

溶剂：DMSO

Ex(nm):491

Em(nm):516

产品介绍

四嗪-反式-环辛烯（TCO）的连接构成了快速的生物分子标记方法。四嗪功能化的分子与TCO功能化的分子反应，通过二氢吡嗪部分形成稳定的结合物。由于其极快的动力学，它已获得普及。AAT Bioquest提供了一组含四嗪和TCO的染料，用于探索可以利用这种强大点击反应的各种生物系统。iFluor™488-TCO可以轻松用于标记四嗪改性的生物分子，用于荧光成像和其他基于荧光的生化分析。与荧光素衍生物（例如FITC）的缀合物相比，使用iFluor™488染料制备的缀合物要优越得多。iFluor™488缀合物比荧光素缀合物明显更亮，并且光稳定性更高。此外，iFluor™488的荧光不受pH（4-10）的影响。这种pH不敏感性是对荧光素的一个重大改进，荧光素仅在pH高于9时才发出大荧光。iFluor™488具有类似于AlexaFluor®488的光谱特性，在某些情况下，它显示出更明亮的信号（AlexaFluor®是Invitrogen的商标）。

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参考文献

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