生物荧光素 CAS 134759-22-1

产品基本信息

产品名称：生物荧光素

CAS：134759-22-1

储存条件：-15℃避光防潮

保质期：12个月

产品物理化学光谱特性

分子量：831.01

溶剂：DMSO

激发波长（nm）：492

发射波长（nm）：515

产品介绍

生物荧光素是美国AAT Bioquest生产的荧光染料。该双功能生物素衍生物用于分光光度测定生物素化程度。该测定基于在生物素存在下链霉抗生物素蛋白/荧光素生物素复合物的荧光增强的动力学分析。荧光素生物素的荧光增强与生物素的浓度成比例。因为该测定适用于5-50uL的小体积或基于珠子的测定，所以检测限可以扩展到飞摩尔范围。动态方面允许将测定扩展到更广泛的应用，包括其用作在微流体装置中进行的层流测定中的试剂混合的指示剂。百萤生物是AAT Bioquest的中国代理商，为您提供优质的生物荧光素。 

点击查看光谱

参考文献

Dynamics of fluorescence dequenching of ostrich-quenched fluorescein biotin: a multifunctional quantitative assay for biotin
Authors: Wu Y, Simons PC, Lopez GP, Sklar LA, Buranda T.
Journal: Anal Biochem (2005): 221

Electrophoretic behavior of streptavidin complexed to a biotinylated probe: a functional screening assay for biotin-binding proteins
Authors: Humbert N, Zocchi A, Ward TR.
Journal: Electrophoresis (2005): 47

Engineered single-chain dimeric streptavidins with an unexpected strong preference for biotin-4-fluorescein
Authors: Aslan FM, Yu Y, Mohr SC, Cantor CR.
Journal: Proc Natl Acad Sci U S A (2005): 8507

Preparation and characterisation of antibody modified gelatin nanoparticles as drug carrier system for uptake in lymphocytes
Authors: Balthasar S, Michaelis K, Dinauer N, von Briesen H, Kreuter J, Langer K.
Journal: Biomaterials (2005): 2723

Controlled surface functionalization of silica nanospheres by covalent conjugation reactions and preparation of high density streptavidin nanoparticles
Authors: Schiestel T, Brunner H, Tovar GE.
Journal: J Nanosci Nanotechnol (2004): 504

Synthesis of adenosine derivatives as transcription initiators and preparation of 5' fluorescein- and biotin-labeled RNA through one-step in vitro transcription
Authors: Huang F, Wang G, Coleman T, Li N.
Journal: Rna (2003): 1562

A novel intravascular drug delivery method using endothelial biotinylation and avidin-biotin binding
Authors: Hoya K, Guterman LR, Miskolczi L, Hopkins LN.
Journal: Drug Deliv (2001): 215

Organelle pH studies using targeted avidin and fluorescein-biotin
Authors: Wu MM, Llopis J, Adams S, McCaffery JM, Kulomaa MS, Machen TE, Moore HP, Tsien RY.
Journal: Chem Biol (2000): 197

Accurate measurement of avidin and streptavidin in crude biofluids with a new, optimized biotin-fluorescein conjugate
Authors: Kada G, Falk H, Gruber HJ.
Journal: Biochim Biophys Acta (1999): 33

Rapid estimation of avidin and streptavidin by fluorescence quenching or fluorescence polarization
Authors: Kada G, Kaiser K, Falk H, Gruber HJ.
Journal: Biochim Biophys Acta (1999): 44