Cell Navigator™TMR神经酰胺高尔基体染色试剂盒*红色荧光*

高尔基体是大多数真核细胞胞质内囊泡和折叠膜的复合体，参与分泌和细胞内运输。它修饰内质网（ER）中内置的蛋白质和脂质，并准备将其运输到细胞外。它还在脂质在整个细胞中的运输和溶酶体的形成中起着重要作用。Cell Navigator™TMR神经酰胺高尔基染色试剂盒提供了一种简便，快速的方法，可以在活细胞中以红色荧光染色高尔基体。高尔基体通过形成相应的荧光代谢产物而被染色。Cell Navigator™TMR神经酰胺高尔基染色试剂盒提供了一种优化的测定方法，可用于通过荧光显微镜检查高尔基体的形态。

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荧光显微镜
Ex:	Cy3/TRITC滤波片组
Em：	Cy3/TRITC 滤波片组
推荐孔板:	黑色透明底板

样品分析方案

概述

根据需要处理细胞

加入GGR169-神经酰胺工作溶液并在室温或37°C下孵育15至30分钟

添加染色缓冲液

使用Cy3滤波片组在显微镜下观察 

 

储备溶液配制

 

操作步骤

 

图示

图1.HeLa细胞中GGR169神经酰胺高尔基染色的荧光图像。在37°C下，用100μL具有Hoechst 33342的GGR169-神经酰胺工作溶液对细胞染色20分钟。

 

参考文献

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Ceramide Transporter CERT Is Involved in Muscle Insulin Signaling Defects Under Lipotoxic Conditions.
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Ceramide-transfer protein-mediated ceramide transfer is a structurally tunable flow-inducing mechanism with structural feed-forward loops.
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