6-HEX亚磷酰胺单体 [5’-六氯荧光素氨基磷酸酯] CAS 1360547-55-2
Ex (nm) | 533 | Em (nm) | 559 |
分子量 | 1050.61 | 溶剂 | MeCN |
存储条件 | 在零下15度以下保存, 避免光照 |
产品基本信息
产品名称:6-HEX氨基磷酸酯[5’-六氯荧光素氨基磷酸酯]
CAS:1360547-55-2
储存条件:-15℃干燥
保质期:12个月
产品物理化学光谱特性
分子量:1050.61
外观:紫色固体
溶剂:MeCN
激发波长(nm):535
发射波长(nm):553
注:该产品是非荧光的,如需PCR荧光检测,请使用#202
产品介绍
6-HEX亚磷酰胺单体 5’-六氯荧光素氨基磷酸酯是美国AAT Bioquest生产的荧光染料,有几种用荧光素标记寡核苷酸的方法。根据光谱要求,标签的选择进一步多样化。5'-荧光素-CE亚磷酰胺,衍生自单一异构体6-羧基荧光素,5'-六氯荧光素-CE亚磷酰胺(HEX)和5'-四氯荧光素-CE亚磷酰胺(TET)均可用于有效标记寡核苷酸。用荧光素标记寡核苷酸的3'末端可以使用3'-荧光素CPG实现。当使用任何这些载体时,标准裂解和用氢氧化铵去保护释放荧光素标记的寡核苷酸。百萤生物是AAT Bioquest 的中国代理商,为您提供优质的6-HEX亚磷酰胺单体 5’-六氯荧光素氨基磷酸酯。
参考文献
Sequence-specific, self-reporting hairpin inversion probes
Authors: Browne KA.
Journal: J Am Chem Soc (2005): 1989
Modified oligonucleotides containing lithocholic acid in their backbones: their enhanced cellular uptake and their mimicking of hairpin structures
Authors: Kim SJ, Bang EK, Kwon HJ, Shim JS, Kim BH.
Journal: Chembiochem (2004): 1517
Synthesis of HyBeacons and dual-labelled probes containing 2'-fluorescent groups for use in genetic analysis
Authors: Dobson N, McDowell DG, French DJ, Brown LJ, Mellor JM, Brown T.
Journal: Chem Commun (Camb) (2003): 1234
Reduced aggregation and improved specificity of G-rich oligodeoxyribonucleotides containing pyrazolo[3,4-d]pyrimidine guanine bases
Authors: Kutyavin IV, Lokhov SG, Afonina IA, Dempcy R, Gall AA, Gorn VV, Lukhtanov E, Metcalf M, Mills A, Reed MW, Sanders S, Shishkina I, Vermeulen NM.
Journal: Nucleic Acids Res (2002): 4952
Diels--Alder bioconjugation of diene-modified oligonucleotides
Authors: Hill KW, Taunton-Rigby J, Carter JD, Kropp E, Vagle K, Pieken W, McGee DP, Husar GM, Leuck M, Anziano DJ, Sebesta DP.
Journal: J Org Chem (2001): 5352
Recovery of an oligonucleotide using silver ions immobilized onto paramagnetic particles
Authors: Ramirez-Vick JE, Garcia AA, Lee J.
Journal: Prep Biochem Biotechnol (1998): 243
Simultaneous detection of several oligonucleotides by time-resolved fluorometry: the use of a mixture of categorized microparticles in a sandwich type mixed-phase hybridization assay
Authors: Hakala H, Virta P, Salo H, Lonnberg H.
Journal: Nucleic Acids Res (1998): 5581
Synthesis and characterization of a new 5-thiol-protected deoxyuridine phosphoramidite for site-specific modification of DNA
Authors: Meyer KL, Hanna MM.
Journal: Bioconjug Chem (1996): 401
Characterization of endonucleolytic activity of HIV-1 integrase using a fluorogenic substrate
Authors: Lee SP, Censullo ML, Kim HG, Knutson JR, Han MK.
Journal: Anal Biochem (1995): 295
Synthesis and characterisation of fluorescent oligonucleotides. Effect of internal labelling on protein recognition
Authors: Hagmar P, Bailey M, Tong G, Haralambidis J, Sawyer WH, Davidson BE.
Journal: Biochim Biophys Acta (1995): 259