6-FAM亚磷酰胺单体 5’-荧光素氨基磷酸酯 CAS 204697-37-0

产品基本信息

产品名称：6-FAM亚磷酰胺单体 5’-荧光素氨基磷酸酯

CAS：204697-37-0

储存条件：-15℃避光防潮

保质期：6个月

 

产品物理化学光谱特性

分子量：843.94

溶剂：MeCN

激发波长（nm）：494

发射波长（nm）：522

 

产品介绍

6-FAM亚磷酰胺单体 5’-荧光素氨基磷酸酯是美国AAT Bioquest生产的荧光染料。有几种用荧光素标记寡核苷酸的方法。根据光谱要求，标签的选择进一步多样化。5'-荧光素-CE亚磷酰胺，衍生自单一异构体6-羧基荧光素，5'-六氯荧光素-CE亚磷酰胺（HEX）和5'-四氯荧光素-CE亚磷酰胺（TET）均可用于有效标记寡核苷酸。 '-结束。用荧光素标记寡核苷酸的3'末端可以使用3'-荧光素CPG实现。当使用任何这些载体时，用氢氧化铵进行标准裂解和去保护释放荧光素标记的寡核苷酸。百萤生物是AAT Bioquest的中国代理商，为您提供优质的6-FAM亚磷酰胺单体 5’-荧光素氨基磷酸酯。 

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参考文献

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Modified oligonucleotides containing lithocholic acid in their backbones: their enhanced cellular uptake and their mimicking of hairpin structures
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Synthesis of HyBeacons and dual-labelled probes containing 2'-fluorescent groups for use in genetic analysis
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Reduced aggregation and improved specificity of G-rich oligodeoxyribonucleotides containing pyrazolo[3,4-d]pyrimidine guanine bases
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Journal: Nucleic Acids Res (2002): 4952

Diels--Alder bioconjugation of diene-modified oligonucleotides
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Journal: J Org Chem (2001): 5352

Recovery of an oligonucleotide using silver ions immobilized onto paramagnetic particles
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Journal: Prep Biochem Biotechnol (1998): 243

Simultaneous detection of several oligonucleotides by time-resolved fluorometry: the use of a mixture of categorized microparticles in a sandwich type mixed-phase hybridization assay
Authors: Hakala H, Virta P, Salo H, Lonnberg H.
Journal: Nucleic Acids Res (1998): 5581

Synthesis and characterization of a new 5-thiol-protected deoxyuridine phosphoramidite for site-specific modification of DNA
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Journal: Bioconjug Chem (1996): 401

Characterization of endonucleolytic activity of HIV-1 integrase using a fluorogenic substrate
Authors: Lee SP, Censullo ML, Kim HG, Knutson JR, Han MK.
Journal: Anal Biochem (1995): 295

Synthesis and characterisation of fluorescent oligonucleotides. Effect of internal labelling on protein recognition
Authors: Hagmar P, Bailey M, Tong G, Haralambidis J, Sawyer WH, Davidson BE.
Journal: Biochim Biophys Acta (1995): 259

 

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