7-羟基-4-甲基香豆素 CAS 90-33-5
英文名称：7-Hydroxy-4-methylcoumarin [4-Methylumbelliferone] *Fluorescence reference standard*
产品参数
| Ex (nm) | 360 | Em (nm) | 448 |
| 分子量 | 176.17 | 溶剂 | DMSO |
| 存储条件 | 在零下15度以下保存, 避免光照 |
产品概述
产品基本信息
产品名称：7-羟基-4-甲基香豆素*荧光参照标准*
CAS：90-33-5
储存条件：-15℃避光防潮
保质期：12个月
产品物理化学光谱特性
分子量：176.17
外观：灰白色粉末
溶剂：DMSO
激发波长(nm)：360
发射波长(nm)：448
产品介绍
该化合物用作基于7-羟基-4-甲基香豆素的酶底物的校准标准。 它具有pH依赖性荧光。
适用仪器
| 荧光酶标仪 | |
| Ex: | 586nm |
| Em： | 605nm |
| Cutoff: | 590nm |
| 推荐孔板: | 纯黑色孔板 |
| 滤波片组: | TRITC |
溶解方案(溶剂以说明书为准)
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 567.634 µL | 2.838 mL | 5.676 mL | 28.382 mL | 56.763 mL |
| 5 mM | 113.527 µL | 567.634 µL | 1.135 mL | 5.676 mL | 11.353 mL |
| 10 mM | 56.763 µL | 283.817 µL | 567.634 µL | 2.838 mL | 5.676 mL |
参考文献
Excited state properties of 7-hydroxy-4-methylcoumarin in the gas phase and in solution. A theoretical study
Authors: Georgieva I, Trendafilova N, Aquino A, Lischka H.
Journal: J Phys Chem A Mol Spectrosc Kinet Environ Gen Theory (2005): 11860
Authors: Georgieva I, Trendafilova N, Aquino A, Lischka H.
Journal: J Phys Chem A Mol Spectrosc Kinet Environ Gen Theory (2005): 11860
Novel thiocoumarins as inhibitors of TNF-alpha induced ICAM-1 expression on human umbilical vein endothelial cells (HUVECs) and microsomal lipid peroxidation
Authors: Kumar S, Singh BK, Kalra N, Kumar V, Kumar A, Prasad AK, Raj HG, Parmar VS, Ghosh B.
Journal: Bioorg Med Chem (2005): 1605
Authors: Kumar S, Singh BK, Kalra N, Kumar V, Kumar A, Prasad AK, Raj HG, Parmar VS, Ghosh B.
Journal: Bioorg Med Chem (2005): 1605
Stability of the complexes of some lanthanides with coumarin derivatives. I. Cerium(III)-4-methyl-7-hydroxycoumarin
Authors: Kostova IP, Manolov, II, Radulova MK.
Journal: Acta Pharm (2004): 37
Authors: Kostova IP, Manolov, II, Radulova MK.
Journal: Acta Pharm (2004): 37
Measurement of enzyme kinetics using microscale steady-state kinetic analysis
Authors: Gleason NJ, Carbeck JD.
Journal: Langmuir (2004): 6374
Authors: Gleason NJ, Carbeck JD.
Journal: Langmuir (2004): 6374
Structural requirements of hydroxylated coumarins for in vitro anti-Helicobacter pylori activity
Authors: Kawase M, Tanaka T, Sohara Y, Tani S, Sakagami H, Hauer H, Chatterjee SS.
Journal: In Vivo (2003): 509
Authors: Kawase M, Tanaka T, Sohara Y, Tani S, Sakagami H, Hauer H, Chatterjee SS.
Journal: In Vivo (2003): 509
Crystal structure of 7,8-dihydroxy-4-methylcoumarin
Authors: Kurosaki H, Sharma RK, Otsuka M, Goto M.
Journal: Anal Sci (2003): 647
Authors: Kurosaki H, Sharma RK, Otsuka M, Goto M.
Journal: Anal Sci (2003): 647
Modifying rates of reductive elimination of leaving groups from indolequinone prodrugs: a key factor in controlling hypoxia-selective drug release
Authors: Everett SA, Swann E, Naylor MA, Stratford MR, Patel KB, Tian N, Newman RG, Vojnovic B, Moody CJ, Wardman P.
Journal: Biochem Pharmacol (2002): 1629
Authors: Everett SA, Swann E, Naylor MA, Stratford MR, Patel KB, Tian N, Newman RG, Vojnovic B, Moody CJ, Wardman P.
Journal: Biochem Pharmacol (2002): 1629
Effects of 18 alpha-glycyrrhizic acid on rat liver cytochrome P450 isoenzymes and phase II transferase
Authors: Yang J, Peng RX, Kong R, Yu JP.
Journal: Yao Xue Xue Bao (2001): 321
Authors: Yang J, Peng RX, Kong R, Yu JP.
Journal: Yao Xue Xue Bao (2001): 321
Synthesis and evaluation of novel fluorogenic substrates for the detection of bacterial beta-galactosidase
Authors: Chilvers KF, Perry JD, James AL, Reed RH.
Journal: J Appl Microbiol (2001): 1118
Authors: Chilvers KF, Perry JD, James AL, Reed RH.
Journal: J Appl Microbiol (2001): 1118
Antioxidative and prooxidative effects of coumarin derivatives on free radical initiated and photosensitized peroxidation of human low-density lipoprotein
Authors: Liu ZQ, Yu W, Liu ZL.
Journal: Chem Phys Lipids (1999): 125
Authors: Liu ZQ, Yu W, Liu ZL.
Journal: Chem Phys Lipids (1999): 125
