Cy5生物素缀合物
英文名称：Cy5 biotin conjugate
产品参数
| Ex (nm) | 651 | Em (nm) | 670 |
| 分子量 | 1353.82 | 溶剂 | Water |
| 存储条件 | 在零下15度以下保存, 避免光照 |
产品概述
产品基本信息
产品名称：Cy5生物素缀合物
储存条件：-15℃避光防潮
保质期：12个月
产品物理化学光谱特性
分子量：1179.49
外观：固体
溶剂：水
激发波长（nm）：649
发射波长（nm）：665
产品介绍
这种双功能Cy5生物素缀合物显示出优异的抗生物素结合和强荧光。它具有与天然生物素相似的抗生物素结合特性，在高亲和力、快速结合以及与抗生物素和链霉亲和素四聚体的非协同结合方面。Cy5生物素可用于检测和定量抗生物素、链霉抗生物素或中微体抗生物素的生物素结合位点。该试剂克服了常用的生物素-4-荧光素的主要缺点，例如在水性介质中的溶解度差以及pH依赖性的吸收和发射。Cy5生物素是一种水溶性试剂，其红色荧光不依赖于pH 4至pH 10。生物素部分和荧光标记之间的柔性PEG间隔物将与抗生物素、链霉抗生物素或中性抗生物素结合所涉及的空间位阻降至最低。
试剂应用文献
Streptavidin cooperative allosterism upon binding biotin observed by differential changes in intrinsic fluorescence
Authors: Waner, M. J., Hiznay, J. M., Mustovich, A. T., Patton, W., Ponyik, C., Mascotti, D. P.
Journal: Biochem Biophys Rep (2019): 127-131
Authors: Waner, M. J., Hiznay, J. M., Mustovich, A. T., Patton, W., Ponyik, C., Mascotti, D. P.
Journal: Biochem Biophys Rep (2019): 127-131
Biotin-tagged fluorescent sensor to visualize 'mobile' Zn(2+) in cancer cells
Authors: Fang, L., Trigiante, G., Kousseff, C. J., Crespo-Otero, R., Philpott, M. P., Watkinson, M.
Journal: Chem Commun (Camb) (2018): 9619-9622
Authors: Fang, L., Trigiante, G., Kousseff, C. J., Crespo-Otero, R., Philpott, M. P., Watkinson, M.
Journal: Chem Commun (Camb) (2018): 9619-9622
Synthesis of Two-Photon Active Tricomponent Fluorescent Probe for Distinguishment of Biotin Receptor Positive and Negative Cells and Imaging 3D-Spheroid
Authors: Pal, K., Sharma, A., Koner, A. L.
Journal: Org Lett (2018): 6425-6429
Authors: Pal, K., Sharma, A., Koner, A. L.
Journal: Org Lett (2018): 6425-6429
A green fluorescent protein-based assay for high-throughput ligand-binding studies of a mycobacterial biotin protein ligase
Authors: Bond, T. E. H., Sorenson, A. E., Schaeffer, P. M.
Journal: Microbiol Res (2017): 35-39
Authors: Bond, T. E. H., Sorenson, A. E., Schaeffer, P. M.
Journal: Microbiol Res (2017): 35-39
Labeling of Cytoskeletal Proteins in Living Cells Using Biotin Ligase Carrying a Fluorescent Protein
Authors: Nishi, S., Yamamoto, C., Yoneda, S., Sueda, S.
Journal: Anal Sci (2017): 897-902
Authors: Nishi, S., Yamamoto, C., Yoneda, S., Sueda, S.
Journal: Anal Sci (2017): 897-902
Determination of Biotin in Infant, Pediatric, and Adult Nutritionals by High-Performance Liquid Chromatography and Fluorescence Detection: Single-Laboratory Validation, First Action 2016.11
Authors: Lin, Q., Ding, Y., Poh, F., Zhang, C., Pan, S. J., Schimpf, K. J.
Journal: J AOAC Int (2017): 145-151
Authors: Lin, Q., Ding, Y., Poh, F., Zhang, C., Pan, S. J., Schimpf, K. J.
Journal: J AOAC Int (2017): 145-151
Quantum dot-fluorescence in situ hybridisation for Ectromelia virus detection based on biotin-streptavidin interactions
Authors: Wang, T., Zheng, Z., Zhang, X. E., Wang, H.
Journal: Talanta (2016): 179-184
Authors: Wang, T., Zheng, Z., Zhang, X. E., Wang, H.
Journal: Talanta (2016): 179-184
Oligonucleotide-stabilized fluorescent silver nanoclusters for the specific and sensitive detection of biotin
Authors: Xiong, X., Tang, Y., Zhao, J., Zhao, S.
Journal: Analyst (2016): 1499-505
Authors: Xiong, X., Tang, Y., Zhao, J., Zhao, S.
Journal: Analyst (2016): 1499-505
Highly Selective Fluorescent Turn-On Probe for Protein Thiols in Biotin Receptor-Positive Cancer Cells
Authors: Sun, Q., Sun, D., Song, L., Chen, Z., Chen, Z., Zhang, W., Qian, J.
Journal: Anal Chem (2016): 3400-5
Authors: Sun, Q., Sun, D., Song, L., Chen, Z., Chen, Z., Zhang, W., Qian, J.
Journal: Anal Chem (2016): 3400-5
A biotin-conjugated glutathione-responsive FRET-based fluorescent probe with a ferrocenyl BODIPY as the dark quencher
Authors: Shi, W. J., Lo, P. C., Zhao, S., Wong, R. C., Wang, Q., Fong, W. P., Ng, D. K.
Journal: Dalton Trans (2016): 17798-17806
Authors: Shi, W. J., Lo, P. C., Zhao, S., Wong, R. C., Wang, Q., Fong, W. P., Ng, D. K.
Journal: Dalton Trans (2016): 17798-17806
参考文献
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Evaluation of a new biotin-DOTA conjugate for pretargeted antibody-guided radioimmunotherapy (PAGRIT((R)))
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Journal: Eur J Nucl Med Mol Imaging. (2006)
Authors: Urbano N, Papi S, Ginanneschi M, De Santis R, Pace S, Lindstedt R, Ferrari L, Choi S, Paganelli G, Chinol M.
Journal: Eur J Nucl Med Mol Imaging. (2006)
A biotin-protein bond with stability in plasma
Authors: Bogusiewicz A, Mock NI, Mock DM.
Journal: Anal Biochem (2005): 98
Authors: Bogusiewicz A, Mock NI, Mock DM.
Journal: Anal Biochem (2005): 98
Instability of the biotin-protein bond in human plasma
Authors: Bogusiewicz A, Mock NI, Mock DM.
Journal: Anal Biochem (2004): 156
Authors: Bogusiewicz A, Mock NI, Mock DM.
Journal: Anal Biochem (2004): 156
Design, synthesis, and evaluation of 5'-S-aminoethyl-N(6)- azidobenzyl-5'-thioadenosine biotin conjugate: a bifunctional photoaffinity probe for the es nucleoside transporter
Authors: Addo JK, Buolamwini JK.
Journal: Bioconjug Chem (2004): 536
Authors: Addo JK, Buolamwini JK.
Journal: Bioconjug Chem (2004): 536
Biotin is endogenously expressed in select regions of the rat central nervous system
Authors: McKay BE, Molineux ML, Turner RW.
Journal: J Comp Neurol (2004): 86
Authors: McKay BE, Molineux ML, Turner RW.
Journal: J Comp Neurol (2004): 86
Probing the interaction of the biotin-avidin complex with the relaxivity of biotinylated Gd-DTPA
Authors: Langereis S, Kooistra HA, van Genderen MH, Meijer EW.
Journal: Org Biomol Chem (2004): 1271
Authors: Langereis S, Kooistra HA, van Genderen MH, Meijer EW.
Journal: Org Biomol Chem (2004): 1271
Immunoassay of total prostate-specific antigen using europium(III) nanoparticle labels and streptavidin-biotin technology
Authors: Huhtinen P, Soukka T, Lovgren T, Harma H.
Journal: J Immunol Methods (2004): 111
Authors: Huhtinen P, Soukka T, Lovgren T, Harma H.
Journal: J Immunol Methods (2004): 111
Volume of RBCs, 24- and 48-hour posttransfusion survivals, and the lifespan of (51)Cr and biotin-X-N-hydroxysuccinimide (NHS)-labeled autologous baboon RBCs: effect of the anticoagulant and blood pH on (51)Cr and biotin-X-NHS elution in vivo
Authors: Valeri CR, Pivacek LE, Cassidy GP, Ragno G.
Journal: Transfusion (2002): 343
Authors: Valeri CR, Pivacek LE, Cassidy GP, Ragno G.
Journal: Transfusion (2002): 343
