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生物素-4-荧光素 CAS 1032732-74-3

英文名称:Biotin-4-fluorescein *CAS 1032732-74-3*
产品参数
Ex (nm)498Em (nm)517
分子量644.69溶剂DMSO
存储条件在零下15度以下保存, 避免光照
产品概述

产品基本信息

产品名称:生物素-4-荧光素 

CAS:1032732-74-3

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:644.69

溶剂:DMSO

激发波长(nm):492

发射波长(nm):518

 

适用仪器

荧光酶标仪  
Ex: 492nm
Em: 518nm
cutoff: 500nm
推荐孔板: 纯黑色孔板

 

试剂应用文献

Nanobody production can be simplified by direct secretion from Escherichia coli
Authors: Iwaki, Takayuki and Hara, Kimiko and Umemura, Kazuo
Journal: Protein Expression and Purification (2020): 105607

 

参考文献

Engineered single-chain dimeric streptavidins with an unexpected strong preference for biotin-4-fluorescein
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Dynamics of fluorescence dequenching of ostrich-quenched fluorescein biotin: a multifunctional quantitative assay for biotin
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Preparation and characterisation of antibody modified gelatin nanoparticles as drug carrier system for uptake in lymphocytes
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Electrophoretic behavior of streptavidin complexed to a biotinylated probe: a functional screening assay for biotin-binding proteins
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Journal: Electrophoresis (2005): 47
 
Controlled surface functionalization of silica nanospheres by covalent conjugation reactions and preparation of high density streptavidin nanoparticles
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Synthesis of adenosine derivatives as transcription initiators and preparation of 5' fluorescein- and biotin-labeled RNA through one-step in vitro transcription
Authors: Huang F, Wang G, Coleman T, Li N.
Journal: Rna (2003): 1562
 
A novel intravascular drug delivery method using endothelial biotinylation and avidin-biotin binding
Authors: Hoya K, Guterman LR, Miskolczi L, Hopkins LN.
Journal: Drug Deliv (2001): 215
 
Organelle pH studies using targeted avidin and fluorescein-biotin
Authors: Wu MM, Llopis J, Adams S, McCaffery JM, Kulomaa MS, Machen TE, Moore HP, Tsien RY.
Journal: Chem Biol (2000): 197
 
Accurate measurement of avidin and streptavidin in crude biofluids with a new, optimized biotin-fluorescein conjugate
Authors: Kada G, Falk H, Gruber HJ.
Journal: Biochim Biophys Acta (1999): 33
 
Rapid estimation of avidin and streptavidin by fluorescence quenching or fluorescence polarization
Authors: Kada G, Kaiser K, Falk H, Gruber HJ.
Journal: Biochim Biophys Acta (1999): 44