钙黄绿素 CAS 154071-48-4

产品基本信息

产品名称：钙黄绿素

CAS：154071-48-4

储存条件：-15℃避光防潮

保质期：24个月

 

产品物理化学光谱特性

分子量：622.53

溶剂：DMSO

激发波长(nm)：495

发射波长(nm)：515

 

选择指南

Dye		Calcein Blue	CytoCalcein™ Violet 450	Calcein UltraBlue™	CytoCalcein™ Violet 500	Calcein UltraGreen™	Calcein	Calcein Orange™	Calcein Red™	Calcein Deep Red™
原理		在活细胞中，非荧光钙黄绿素 AM 在细胞内酯酶的作用下 AM 酯水解后转化为荧光钙黄绿素产物。
荧光效果		活细胞具有高度荧光，很容易与无荧光的死细胞区分开。
Laser(nm)		UV (350)	Violet (405)	UV (350)	Violet (405)	Blue (488)	Blue (488)	Green (532)	Yellow (561-568)	Red (633-647)
Ex/Em(nm)		354/441	406/445	359/458	420/505	492/514	501/521	531/545	562/576	643/663
是否复用		是	是	是	是	是	是	是	是	是
染料相容性		与绿色和红色荧光蛋白、指示剂和其他探针兼容	与绿色和红色荧光蛋白、指示剂和其他探针兼容	与绿色和红色荧光蛋白、指示剂和其他探针兼容	与蓝色和红色荧光蛋白、指示剂和其他探针兼容	与蓝色和红色荧光蛋白、指示剂和其他探针兼容	与蓝色和红色荧光蛋白、指示剂和其他探针兼容	与蓝色和红色荧光蛋白、指示剂和其他探针兼容	与蓝色和绿色荧光蛋白、指示剂和其他探针兼容	与蓝色和绿色荧光蛋白、指示剂和其他探针兼容
样品类型		活细胞
适用仪器		荧光显微镜、荧光酶标仪、流式细胞仪
货号		22007	22012	21908	22013	21905	22003	22009	21900	22011

 

产品介绍

钙黄绿素*超纯级*是美国AAT Bioquest生产的钙指示剂，钙黄绿素，也称为荧光素，荧光素复合物，是一种荧光钙指示剂，其激发和发射波长分别为495和515nm。钙黄绿素在100 mM以上的浓度下会自动猝灭。它用作EDTA滴定钙离子和荧光测定钙的络合指示剂，微溶于水。质子解离常数为pKa1的= 2.1，pKa2 = 2.9，pKa3 = 4.2，pKa4 = 5.5，pKa5 = 10.8和pKa6 = 11.7。钙黄绿素溶液在酸性条件下发出黄绿色荧光，而在碱性条件下不发出荧光。但是，钙黄绿素在碱性条件下在金属离子（例如Al，Ba，Cu，Mg和Zn）存在下发出荧光。因此，钙黄绿素还可以用于除Ca之外的这些重金属离子的直接荧光滴定。百萤生物是AAT Bioquest的中国代理商，为您提供优质的钙黄绿素*超纯级*。 

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参考文献

Functional imaging of neuronal activity of auditory cortex by using Cal-520 in anesthetized and awake mice
Authors: Jingcheng Li, Jianxiong Zhang, Meng Wang, Junxia Pan, Xiaowei Chen, Xiang Liao
Journal: Biomedical Optics Express (2017): 2599--2610

NINJ2--A novel regulator of endothelial inflammation and activation
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Erythropoietin Stimulates Endothelial Progenitor Cells to Induce Endothelialization in an Aneurysm Neck After Coil Embolization by Modulating Vascular Endothelial Growth Factor
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Flexible Endoscopic Spray Application of Respiratory Epithelial Cells as Platform Technology to Apply Cells in Tubular Organs
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Journal: Tissue Engineering Part C: Methods (2016): 322--331

Influence of hypothermia and subsequent rewarming upon leukocyte-endothelial interactions and expression of Junctional-Adhesion-Molecules A and B
Authors: Nicolai V Bogert, Isabella Werner, Angela Kornberger, Patrick Meybohm, Anton Moritz, Till Keller, Ulrich A Stock, Andres Beiras-Fernandez
Journal: Scientific reports (2016)

Inhibition of ABC transport proteins by oil sands process affected water
Authors: Hattan A Alharbi, David MV Saunders, Ahmed Al-Mousa, Jane Alcorn, Alberto S Pereira, Jonathan W Martin, John P Giesy, Steve B Wiseman
Journal: Aquatic Toxicology (2016): 81--88

Rapid generation of collagen-based microtissues to study cell--matrix interactions
Authors: Marie-Elena Brett, Alexandra L Crampton, David K Wood
Journal: Technology (2016): 1--8

Toxicokinetics and toxicodynamics of chlorpyrifos is altered in embryos of Japanese medaka exposed to oil sands process-affected water: evidence for inhibition of P-glycoprotein
Authors: Hattan A Alharbi, Jane Alcorn, Ahmed Al-Mousa, John P Giesy, Steve B Wiseman
Journal: Journal of Applied Toxicology (2016)

Spraying respiratory epithelial cells to coat tissue-engineered constructs
Authors: Anja Lena Thiebes, Stefanie Albers, Christian Klopsch, Stefan Jockenhoevel, Christian G Cornelissen
Journal: BioResearch open access (2015): 278--287

The efficient hemostatic effect of Antarctic krill chitosan is related to its hydration property
Authors: Shuai Wu, Zhuoyao Huang, Jianhui Yue, Di Liu, Ting Wang, Pierre Ezanno, Changshun Ruan, Xiaoli Zhao, William W Lu, Haobo Pan
Journal: Carbohydrate polymers (2015): 295--303

 

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