细胞核染料Hoechst 33342 CAS 875756-97-1
英文名称：Hoechst 33342 *Ultrapure Grade*
产品参数
| Ex (nm) | 352 | Em (nm) | 454 |
| 分子量 | 561.93 | 溶剂 | Water |
| 存储条件 | 在零下15度以下保存, 避免光照 |
产品概述
产品基本信息
产品名称：Hoechst 33342细胞核染料
CAS：875756-97-1
储存条件：-20℃干燥
保质期：12个月
产品物理化学光谱特性
分子量：561.93
外观：亮黄色粉末
溶剂：水或DMSO
激发波长(nm)：350
发射波长(nm)：461
产品介绍
Hoechst 染料是一系列用于荧光显微镜标记 DNA 的荧光染料。因为这些荧光染料可以标记 DNA，所以它们也常用于可视化细胞核和线粒体。其中两种密切相关的双苯并咪唑衍生物常被使用：Hoechst 33258 和 Hoechst 33342。这两种染料都在大约 350 纳米的紫外线下激发，并在 461 纳米的发射峰处发出蓝色/青色的荧光。Hoechst 染料可以用于活细胞或固定细胞，通常用作另一种核酸染料 DAPI 的替代品。它们之间的主要区别是 Hoechst 33342 添加的乙基使其更亲脂，因此更能穿透完整的细胞膜。在某些应用中，Hoechst 33258 的渗透性较低。这些染料还可以通过绘制标准的发射量与内容曲线来检测样本 DNA 的含量。
溶解方案(溶剂以说明书为准)
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 177.958 µL | 889.791 µL | 1.78 mL | 8.898 mL | 17.796 mL |
| 5 mM | 35.592 µL | 177.958 µL | 355.916 µL | 1.78 mL | 3.559 mL |
| 10 mM | 17.796 µL | 88.979 µL | 177.958 µL | 889.791 µL | 1.78 mL |
实验方案
染色细胞分析方案
以下程序适用于大多数细胞类型。 生长培养基，细胞密度，细胞类型和其他因素可能影响染色。 玻璃器皿上的残留洗涤剂也可能影响许多生物染色，导致在没有细胞存在的溶液中出现明亮染色的物质。
通过离心沉淀细胞并将细胞重悬于缓冲盐溶液或培养基中，经过测试染料的佳结合pH为7.4。培养物中的贴壁细胞可以在盖玻片上或细胞培养孔中原位染色。Hoechst的染色浓度为0.5-5uM，并将其孵育15至60分钟。在初的实验中，好在整个建议范围内尝试几种染料浓度，以确定产生佳染色的浓度。
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参考文献
Usefulness of a triple fluorochrome combination Merocyanine 540/Yo-Pro 1/Hoechst 33342 in assessing membrane stability of viable frozen-thawed spermatozoa from Estonian Holstein AI bulls
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Fatty acid synthase and its mRNA concentrations are decreased at different times following Hoechst 33342-induced apoptosis in BC3H-1 myocytes
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The DNA minor groove binding agents Hoechst 33258 and 33342 enhance recombinant adeno-associated virus (rAAV) transgene expression
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