Cy5 NHS酯 CAS 146368-14-1

产品基本信息

产品名称：Cy5,NHS酯 

CAS：146368-14-1

储存条件：-15℃避光防潮

保质期：12个月

 

产品物理化学光谱特性

分子量：855.07

外观：蓝色固体

溶剂：DMSO

激发波长（nm）：649

发射波长（nm）：665

 

产品介绍

Cy5,NHS（Cy5）常用于荧光成像和其他基于荧光的生化分析。它们广泛用于标记肽，蛋白质和寡核苷酸等。Cy5染料是常见的红色荧光团中的一种。这些多功能荧光团用于生物相关pH的各种应用时可以耐受3-10范围的pH。该染料还具有DMSO耐受性和光稳定性，能够在不损失性能的情况下从储存转移至分析。它的水溶性在测定缓冲液中对有机溶剂的需要。我们的Cy5荧光染料经过QC测试，可确保高水平的发色团和活性染料含量。单活性染料适用于蛋白质和寡核苷酸的靶向-标记，双活性染料更适用于一般标记。建议使用NHS酯染料标记胺基，建议使用马来酰亚胺染料标记硫醇基团。

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图示

图1.将HeLa细胞分别与（Tubulin +）、（Tubulin-）小鼠抗微管蛋白一起孵育，然后与AAT的Cy3山羊抗小鼠IgG缀合物（红色，左）孵育；与Cy3缀合的Jackson山羊抗小鼠IgG孵育（红色，右）。 细胞核用Hoechst 33342（Blue，Cat＃17530）染色。

 

参考文献

A basic protein, N25, from a mollusk modifies calcium carbonate morphology and shell biomineralization
Authors: Dong Yang, Yi Yan, Xue Yang, Jun Liu, Guilan Zheng, Liping Xie, Rongqing Zhang
Journal: Journal of Biological Chemistry (2019): jbc--RA118

A rapid test strip for diagnosing glycosylated hemoglobin (HbA1c) based on fluorescent affinity immunochromatography
Authors: Chaoman Ang, Doudou Lou, Linling Hu, Wei Chen, Yefei Zhu, Zhirui Guo, Ning Gu, Yu Zhang
Journal: Analytical Sciences (2018): 18P135

Cube-shaped theranostic paclitaxel prodrug nanocrystals with surface functionalization of SPC and MPEG-DSPE for imaging and chemotherapy
Authors: Fuqiang Guo, Jiajia Shang, Hai Zhao, Kangrong Lai, Yang Li, Zhongxiong Fan, Zhenqing Hou, Guanghao Su
Journal: Colloids and Surfaces B: Biointerfaces (2017)

Light/magnetic hyperthermia triggered drug released from multi-functional thermo-sensitive magnetoliposomes for precise cancer synergetic theranostics
Authors: Yuxin Guo, Yang Zhang, Jinyuan Ma, Qi Li, Yang Li, Xinyi Zhou, Dan Zhao, Hua Song, Qing Chen, Xuan Zhu
Journal: Journal of Controlled Release (2017)

Thermo-sensitive hydrogel PLGA-PEG-PLGA as a vaccine delivery system for intramuscular immunization
Authors: Xiaoyan Wang, Yu Zhang, Wei Xue, Hong Wang, Xiaozhong Qiu, Zonghua Liu
Journal: Journal of Biomaterials Applications (2017): 923--932

Affinity-Controlled Protein Encapsulation into Sub-30 nm Telodendrimer Nanocarriers by Multivalent and Synergistic Interactions
Authors: Xu Wang, Changying Shi, Li Zhang, Alexa Bodman, Dandan Guo, Lili Wang, Walter A Hall, Stephan Wilkens, Juntao Luo
Journal: Biomaterials (2016)

Carboxymethyl Dextran-Stabilized Polyethylenimine-Poly (epsilon-caprolactone) Nanoparticles-Mediated Modulation of MicroRNA-34a Expression via Small-Molecule Modulator for Hepatocellular Carcinoma Therapy
Authors: Xiongwei Deng, Zhaoxia Yin, Zhixiang Zhou, Yihui Wang, Fang Zhang, Qin Hu, Yishu Yang, Jianqing Lu, Yan Wu, Wang Sheng
Journal: ACS applied materials & interfaces (2016): 17068--17079

Click-electron microscopy for imaging metabolically tagged nonprotein biomolecules
Authors: John T Ngo, Stephen R Adams, Thomas J Deerinck, Daniela Boassa, Frances Rodriguez-Rivera, Sakina F Palida, Carolyn R Bertozzi, Mark H Ellisman, Roger Y Tsien
Journal: Nat Chem Biol (2016): 459--465

Design, synthesis and evaluation of VEGF-siRNA/CRS as a novel vector for gene delivery
Authors: Wen Zhao, Yifan Zhang, Xueyun Jiang, Chunying Cui
Journal: Drug Design, Development and Therapy (2016): 3851

Molecular Basis and Consequences of the Cytochrome c-tRNA Interaction
Authors: Cuiping Liu, Aaron J Stonestrom, Thomas Christian, Jeongsik Yong, Ryuichi Takase, Ya-Ming Hou, Xiaolu Yang
Journal: Journal of Biological Chemistry (2016): 10426--10436

 

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