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发光底物BOC-Leu-Gly-Arg-氨基荧光素

英文名称:BOC-Leu-Gly-Arg-Aminoluciferin
产品参数
Ex (nm)362Em (nm)499
分子量819.87溶剂DMSO
存储条件在零下15度以下保存, 避免光照
产品概述

产品基本信息

产品名称:发光底物BOC-Leu-Gly-Arg-氨基荧光素

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:819.87

溶剂:DMSO

激发波长(nm):N/A

发射波长(nm):560

 

产品介绍

发光底物BOC-Leu-Gly-Arg-氨基荧光素是美国AAT Bioquest生产的荧光染料,这种基于氨基荧光素的探针是补体成分C3 / C5转化酶,凝血因子Xa和大豆胰蛋白酶样酶的发光底物。 它也被来自人类红细胞的高分子量巯基蛋白酶macropain水解。 可以使用我们的萤光素酶测定试剂盒检测蛋白酶释放的D-氨基荧光素。BOC-Leu-Gly-Arg-Aminoluciferin比广泛使用的荧光AMC底物Boc-Leu-Gly-Arg-AMC敏感得多。百萤生物是AAT Bioquest的中国代理商,为您提供优质的发光底物BOC-Leu-Gly-Arg-氨基荧光素。 

 

试剂应用文献

Endotoxin assay by bioluminescence using mutant firefly luciferase
Authors: Kenichi Noda, Hitoshi Goto, Yuji Murakami, Abo Bakr F Ahmed, Akio Kuroda
Journal: Analytical biochemistry (2010): 152--155

 

参考文献

Identification of mutant firefly luciferases that efficiently utilize aminoluciferins
Authors: Harwood KR, Mofford DM, Reddy GR, Miller SC.
Journal: Chem Biol (2011): 1649
 
In vivo imaging of early stage apoptosis by measuring real-time caspase-3/7 activation
Authors: Scabini M, Stellari F, Cappella P, Rizzitano S, Texido G, Pesenti E.
Journal: Apoptosis (2011): 198
 
Aminoluciferins as functional bioluminogenic substrates of firefly luciferase
Authors: Takakura H, Kojima R, Urano Y, Terai T, Hanaoka K, Nagano T.
Journal: Chem Asian J (2011): 1800
 
Noninvasive molecular imaging of apoptosis in vivo using a modified firefly luciferase substrate, Z-DEVD-aminoluciferin
Authors: Hickson J, Ackler S, Klaubert D, Bouska J, Ellis P, Foster K, Oleksijew A, Rodriguez L, Schlessinger S, Wang B, Frost D.
Journal: Cell Death Differ (2010): 1003
 
Robust light emission from cyclic alkylaminoluciferin substrates for firefly luciferase
Authors: Reddy, G. R.; Thompson, W. C.; Miller, S. C.
Journal: J Am Chem Soc (2010): 13586-7
 
Cell-based bioluminescent assays for all three proteasome activities in a homogeneous format
Authors: Moravec RA, O'Brien MA, Daily WJ, Scurria MA, Bernad L, Riss TL.
Journal: Anal Biochem (2009): 294
 
In vivo bioluminescence imaging of furin activity in breast cancer cells using bioluminogenic substrates
Authors: Dragulescu-Andrasi A, Liang G, Rao J.
Journal: Bioconjug Chem (2009): 1660
 
Homogeneous, bioluminescent proteasome assays
Authors: O'Brien MA, Moravec RA, Riss TL, Bulleit RF.
Journal: Methods Mol Biol (2008): 163
 
N-Alkylated 6'-aminoluciferins are bioluminescent substrates for Ultra-Glo and QuantiLum luciferase: new potential scaffolds for bioluminescent assays
Authors: Woodroofe CC, Shultz JW, Wood MG, Osterman J, Cali JJ, Daily WJ, Meisenheimer PL, Klaubert DH.
Journal: Biochemistry (2008): 10383
 
A homogeneous assay to measure live and dead cells in the same sample by detecting different protease markers
Authors: Niles AL, Moravec RA, Eric Hesselberth P, Scurria MA, Daily WJ, Riss TL.
Journal: Anal Biochem (2007): 197

 

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