预活化HRP NHS酯

ReadiUse 预活化的HRP NHS酯是HRP的单NHS酯。 它可用于容易记蛋白质（例如抗体）或具有氨基的其他生物分子。 ReadiUse 预活化HRP NHS酯易于使用。 它可以用于通过简单混合标记抗体，即抗体的碱性溶液（pH 8.5-9.0）可以直接与HRP NHS酯混合，并将反应混合物摇动1-2小时。 在大多数情况下，所得溶液可直接用于ELISA测定而无需进一步纯化。 ReadiUse 预活化的HRP NHS酯提供了强大的方法来执行抗体与HRP的缀合。 任何合适量的抗体（> 1mg / mL）可以容易地与预活化的HRP NHS酯缀合而无需纯化。百萤生物是AAT Bioquest的中国代理商，为您提供优质的预活化HRP NHS酯。 

样品实验方案

1.抗体（IgG）~100μg在20μL~50μL的25mM碳酸氢钠缓冲液（pH = 8.5）中。

2.将IgG溶液转移到HRP-SE小瓶中，充分混合。

3.在室温下反应1~2小时，优选在反应过程中轻轻摇动。

4.加入等体积的100mM Tris-HCl缓冲液（pH = 7.5）+ 0.5％BSA。

5.将混合物储存在4^oC，即可使用。 注意：如果目标蛋白质溶液含有其他蛋白质（如BSA），则纯化蛋白质溶液或干扰标记反应。 注意：需要除去叠氮化钠等小分子。 注意： Tris缓冲液和其他胺类化合物也会干扰标记反应。

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